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Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya
BACKGROUND: The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects’ success and adaptability. However, bacterial comm...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060952/ https://www.ncbi.nlm.nih.gov/pubmed/32185109 http://dx.doi.org/10.7717/peerj.8701 |
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author | Gichuhi, Joseph Sevgan, Subramanian Khamis, Fathiya Van den Berg, Johnnie du Plessis, Hannalene Ekesi, Sunday Herren, Jeremy K. |
author_facet | Gichuhi, Joseph Sevgan, Subramanian Khamis, Fathiya Van den Berg, Johnnie du Plessis, Hannalene Ekesi, Sunday Herren, Jeremy K. |
author_sort | Gichuhi, Joseph |
collection | PubMed |
description | BACKGROUND: The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects’ success and adaptability. However, bacterial communities in S. frugiperda remain poorly studied. METHODS: We investigated the composition, abundance and diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four maize growing regions in Kenya through high throughput sequencing of the bacterial 16S rRNA gene. The population structure of S. frugiperda in Kenya was assessed through amplification of the mitochondrial cytochrome oxidase subunit I gene. RESULTS: We identified Proteobacteria and Firmicutes as the most dominant bacterial phyla and lesser proportions of Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during metamorphosis. However, several bacterial groups were found in both adults and larvae suggesting that they are transmitted across developmental stages. Reads corresponding to several known entomopathogenic bacterial clades as well as the fungal entomopathogen, Metarhizium rileyi, were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya indicated the presence of both “Rice” and “Corn” strains, with a higher prevalence of the “Rice” strain. |
format | Online Article Text |
id | pubmed-7060952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70609522020-03-17 Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya Gichuhi, Joseph Sevgan, Subramanian Khamis, Fathiya Van den Berg, Johnnie du Plessis, Hannalene Ekesi, Sunday Herren, Jeremy K. PeerJ Agricultural Science BACKGROUND: The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects’ success and adaptability. However, bacterial communities in S. frugiperda remain poorly studied. METHODS: We investigated the composition, abundance and diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four maize growing regions in Kenya through high throughput sequencing of the bacterial 16S rRNA gene. The population structure of S. frugiperda in Kenya was assessed through amplification of the mitochondrial cytochrome oxidase subunit I gene. RESULTS: We identified Proteobacteria and Firmicutes as the most dominant bacterial phyla and lesser proportions of Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during metamorphosis. However, several bacterial groups were found in both adults and larvae suggesting that they are transmitted across developmental stages. Reads corresponding to several known entomopathogenic bacterial clades as well as the fungal entomopathogen, Metarhizium rileyi, were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya indicated the presence of both “Rice” and “Corn” strains, with a higher prevalence of the “Rice” strain. PeerJ Inc. 2020-03-05 /pmc/articles/PMC7060952/ /pubmed/32185109 http://dx.doi.org/10.7717/peerj.8701 Text en © 2020 Gichuhi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Gichuhi, Joseph Sevgan, Subramanian Khamis, Fathiya Van den Berg, Johnnie du Plessis, Hannalene Ekesi, Sunday Herren, Jeremy K. Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title | Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title_full | Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title_fullStr | Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title_full_unstemmed | Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title_short | Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya |
title_sort | diversity of fall armyworm, spodoptera frugiperda and their gut bacterial community in kenya |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060952/ https://www.ncbi.nlm.nih.gov/pubmed/32185109 http://dx.doi.org/10.7717/peerj.8701 |
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