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PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells

Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transd...

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Autores principales: Shin, Min Jea, Kim, Dae Won, Choi, Yeon Joo, Cha, Hyun Ju, Lee, Sung Ho, Lee, Sunghou, Park, Jinseu, Han, Kyu Hyung, Eum, Won Sik, Choi, Soo Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061214/
https://www.ncbi.nlm.nih.gov/pubmed/31964467
http://dx.doi.org/10.5483/BMBRep.2020.53.2.180
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author Shin, Min Jea
Kim, Dae Won
Choi, Yeon Joo
Cha, Hyun Ju
Lee, Sung Ho
Lee, Sunghou
Park, Jinseu
Han, Kyu Hyung
Eum, Won Sik
Choi, Soo Young
author_facet Shin, Min Jea
Kim, Dae Won
Choi, Yeon Joo
Cha, Hyun Ju
Lee, Sung Ho
Lee, Sunghou
Park, Jinseu
Han, Kyu Hyung
Eum, Won Sik
Choi, Soo Young
author_sort Shin, Min Jea
collection PubMed
description Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-βB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases.
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spelling pubmed-70612142020-03-19 PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells Shin, Min Jea Kim, Dae Won Choi, Yeon Joo Cha, Hyun Ju Lee, Sung Ho Lee, Sunghou Park, Jinseu Han, Kyu Hyung Eum, Won Sik Choi, Soo Young BMB Rep Article Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-βB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases. Korean Society for Biochemistry and Molecular Biology 2020-02-29 2020-02-29 /pmc/articles/PMC7061214/ /pubmed/31964467 http://dx.doi.org/10.5483/BMBRep.2020.53.2.180 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Shin, Min Jea
Kim, Dae Won
Choi, Yeon Joo
Cha, Hyun Ju
Lee, Sung Ho
Lee, Sunghou
Park, Jinseu
Han, Kyu Hyung
Eum, Won Sik
Choi, Soo Young
PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title_full PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title_fullStr PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title_full_unstemmed PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title_short PEP-1-GLRX1 protein exhibits anti-inflammatory effects by inhibiting the activation of MAPK and NF-κB pathways in Raw 264.7 cells
title_sort pep-1-glrx1 protein exhibits anti-inflammatory effects by inhibiting the activation of mapk and nf-κb pathways in raw 264.7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061214/
https://www.ncbi.nlm.nih.gov/pubmed/31964467
http://dx.doi.org/10.5483/BMBRep.2020.53.2.180
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