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Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering
A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-b...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061548/ https://www.ncbi.nlm.nih.gov/pubmed/32159146 http://dx.doi.org/10.1016/j.mtbio.2019.100011 |
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author | Witte, K. Rodrigo-Navarro, A. Salmeron-Sanchez, M. |
author_facet | Witte, K. Rodrigo-Navarro, A. Salmeron-Sanchez, M. |
author_sort | Witte, K. |
collection | PubMed |
description | A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III(7-10) fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties. |
format | Online Article Text |
id | pubmed-7061548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70615482020-03-10 Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering Witte, K. Rodrigo-Navarro, A. Salmeron-Sanchez, M. Mater Today Bio Full-Length Article A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III(7-10) fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties. Elsevier 2019-06-18 /pmc/articles/PMC7061548/ /pubmed/32159146 http://dx.doi.org/10.1016/j.mtbio.2019.100011 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Full-Length Article Witte, K. Rodrigo-Navarro, A. Salmeron-Sanchez, M. Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title | Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title_full | Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title_fullStr | Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title_full_unstemmed | Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title_short | Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
title_sort | bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering |
topic | Full-Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061548/ https://www.ncbi.nlm.nih.gov/pubmed/32159146 http://dx.doi.org/10.1016/j.mtbio.2019.100011 |
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