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Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study

OBJECTIVE: Osteoarthritis is the most common type of arthritis and one of the leading causes of job loss and motor disabilities. Recently, the involvement of dopaminergic pathways and dopamine receptor genes has been considered in this disease. Therefore, studying and comparing the expression patter...

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Autores principales: Sheikhpour, Mojgan, Eliaspour, Darioush, Arabi, Iraj, Raeissadat, Seyed Ahmad, Lari, Arezou, Seif Barghi, Tohid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061717/
https://www.ncbi.nlm.nih.gov/pubmed/32184581
http://dx.doi.org/10.2147/CIA.S238351
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author Sheikhpour, Mojgan
Eliaspour, Darioush
Arabi, Iraj
Raeissadat, Seyed Ahmad
Lari, Arezou
Seif Barghi, Tohid
author_facet Sheikhpour, Mojgan
Eliaspour, Darioush
Arabi, Iraj
Raeissadat, Seyed Ahmad
Lari, Arezou
Seif Barghi, Tohid
author_sort Sheikhpour, Mojgan
collection PubMed
description OBJECTIVE: Osteoarthritis is the most common type of arthritis and one of the leading causes of job loss and motor disabilities. Recently, the involvement of dopaminergic pathways and dopamine receptor genes has been considered in this disease. Therefore, studying and comparing the expression pattern of these receptor genes can lead to a greater understanding of the pathogenesis of this disease. METHODS: In this research, we used the systems biology approach to investigate the role of the dopaminergic pathway in osteoarthritis. Then the gene expression pattern of dopamine receptor genes was examined in an osteoarthritis patientgroup in comparison with healthy individuals by Real-time PCR method. RESULTS: The analysis of the transcriptome dataset of osteoarthritis identified some genes in the dopaminergic pathway and the six most important genes in this disease are in the network with a significant relationship to dopamine receptors which differentially expressed compared to health groups. Statistical analysis of the case control study showed a significant difference (P-value<0.05) in DRD1 and DRD2 family in the patients in comparison to healthy individuals. DISCUSSION: We attained the significant expression pattern of dopamine receptors in the blood of osteoarthritis patients which could be useful to identify new strategies for the diagnosis, management, or treatment of this disease.
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spelling pubmed-70617172020-03-17 Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study Sheikhpour, Mojgan Eliaspour, Darioush Arabi, Iraj Raeissadat, Seyed Ahmad Lari, Arezou Seif Barghi, Tohid Clin Interv Aging Original Research OBJECTIVE: Osteoarthritis is the most common type of arthritis and one of the leading causes of job loss and motor disabilities. Recently, the involvement of dopaminergic pathways and dopamine receptor genes has been considered in this disease. Therefore, studying and comparing the expression pattern of these receptor genes can lead to a greater understanding of the pathogenesis of this disease. METHODS: In this research, we used the systems biology approach to investigate the role of the dopaminergic pathway in osteoarthritis. Then the gene expression pattern of dopamine receptor genes was examined in an osteoarthritis patientgroup in comparison with healthy individuals by Real-time PCR method. RESULTS: The analysis of the transcriptome dataset of osteoarthritis identified some genes in the dopaminergic pathway and the six most important genes in this disease are in the network with a significant relationship to dopamine receptors which differentially expressed compared to health groups. Statistical analysis of the case control study showed a significant difference (P-value<0.05) in DRD1 and DRD2 family in the patients in comparison to healthy individuals. DISCUSSION: We attained the significant expression pattern of dopamine receptors in the blood of osteoarthritis patients which could be useful to identify new strategies for the diagnosis, management, or treatment of this disease. Dove 2020-03-04 /pmc/articles/PMC7061717/ /pubmed/32184581 http://dx.doi.org/10.2147/CIA.S238351 Text en © 2020 Sheikhpour et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Sheikhpour, Mojgan
Eliaspour, Darioush
Arabi, Iraj
Raeissadat, Seyed Ahmad
Lari, Arezou
Seif Barghi, Tohid
Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title_full Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title_fullStr Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title_full_unstemmed Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title_short Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
title_sort using the systems biology approach and molecular method to investigate the role of the dopaminergic pathway in osteoarthritis: a case control study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061717/
https://www.ncbi.nlm.nih.gov/pubmed/32184581
http://dx.doi.org/10.2147/CIA.S238351
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