Cargando…

Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis

The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase...

Descripción completa

Detalles Bibliográficos
Autores principales: Cifrová, Petra, Oulehlová, Denisa, Kollárová, Eva, Martinek, Jan, Rosero, Amparo, Žárský, Viktor, Schwarzerová, Kateřina, Cvrčková, Fatima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061858/
https://www.ncbi.nlm.nih.gov/pubmed/32194585
http://dx.doi.org/10.3389/fpls.2020.00148
_version_ 1783504453638291456
author Cifrová, Petra
Oulehlová, Denisa
Kollárová, Eva
Martinek, Jan
Rosero, Amparo
Žárský, Viktor
Schwarzerová, Kateřina
Cvrčková, Fatima
author_facet Cifrová, Petra
Oulehlová, Denisa
Kollárová, Eva
Martinek, Jan
Rosero, Amparo
Žárský, Viktor
Schwarzerová, Kateřina
Cvrčková, Fatima
author_sort Cifrová, Petra
collection PubMed
description The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase cotyledon pavement cell lobing, while mutations affecting ARP2/3 subunits exhibit an opposite effect. Here we examine the role of FH1 and the ARP2/3 complex subunit ARPC5 (At4g01710) in epidermal cell morphogenesis with focus on pavement cells and trichomes using a model system of single fh1 and arpc5, as well as double fh1 arpc5 mutants. While cotyledon pavement cell shape in double mutants mostly resembled single arpc5 mutants, analysis of true leaf epidermal morphology, as well as actin and microtubule organization and dynamics, revealed a more complex relationship between the two systems and similar, rather than antagonistic, effects on some parameters. Both fh1 and arpc5 mutations increased actin network density and increased cell shape complexity in pavement cells and trichomes of first true leaves, in contrast to cotyledons. Thus, while the two actin nucleation systems have complementary roles in some aspects of cell morphogenesis in cotyledon pavement cells, they may act in parallel in other cell types and developmental stages.
format Online
Article
Text
id pubmed-7061858
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-70618582020-03-19 Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis Cifrová, Petra Oulehlová, Denisa Kollárová, Eva Martinek, Jan Rosero, Amparo Žárský, Viktor Schwarzerová, Kateřina Cvrčková, Fatima Front Plant Sci Plant Science The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase cotyledon pavement cell lobing, while mutations affecting ARP2/3 subunits exhibit an opposite effect. Here we examine the role of FH1 and the ARP2/3 complex subunit ARPC5 (At4g01710) in epidermal cell morphogenesis with focus on pavement cells and trichomes using a model system of single fh1 and arpc5, as well as double fh1 arpc5 mutants. While cotyledon pavement cell shape in double mutants mostly resembled single arpc5 mutants, analysis of true leaf epidermal morphology, as well as actin and microtubule organization and dynamics, revealed a more complex relationship between the two systems and similar, rather than antagonistic, effects on some parameters. Both fh1 and arpc5 mutations increased actin network density and increased cell shape complexity in pavement cells and trichomes of first true leaves, in contrast to cotyledons. Thus, while the two actin nucleation systems have complementary roles in some aspects of cell morphogenesis in cotyledon pavement cells, they may act in parallel in other cell types and developmental stages. Frontiers Media S.A. 2020-03-02 /pmc/articles/PMC7061858/ /pubmed/32194585 http://dx.doi.org/10.3389/fpls.2020.00148 Text en Copyright © 2020 Cifrová, Oulehlová, Kollárová, Martinek, Rosero, Žárský, Schwarzerová and Cvrčková http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cifrová, Petra
Oulehlová, Denisa
Kollárová, Eva
Martinek, Jan
Rosero, Amparo
Žárský, Viktor
Schwarzerová, Kateřina
Cvrčková, Fatima
Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title_full Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title_fullStr Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title_full_unstemmed Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title_short Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis
title_sort division of labor between two actin nucleators—the formin fh1 and the arp2/3 complex—in arabidopsis epidermal cell morphogenesis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061858/
https://www.ncbi.nlm.nih.gov/pubmed/32194585
http://dx.doi.org/10.3389/fpls.2020.00148
work_keys_str_mv AT cifrovapetra divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT oulehlovadenisa divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT kollarovaeva divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT martinekjan divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT roseroamparo divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT zarskyviktor divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT schwarzerovakaterina divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis
AT cvrckovafatima divisionoflaborbetweentwoactinnucleatorstheforminfh1andthearp23complexinarabidopsisepidermalcellmorphogenesis