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The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the pot...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061907/ https://www.ncbi.nlm.nih.gov/pubmed/32185104 http://dx.doi.org/10.7717/peerj.8664 |
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author | Liu, Xiu Lv, Qiulan Ren, Hongyan Gao, Liu Zhao, Peng Yang, Xiaomin Yang, Guanpin Xu, Daxing Wang, Guangtao Yang, Wan Wang, Pengjun Wang, Zenglan Xing, Shichao |
author_facet | Liu, Xiu Lv, Qiulan Ren, Hongyan Gao, Liu Zhao, Peng Yang, Xiaomin Yang, Guanpin Xu, Daxing Wang, Guangtao Yang, Wan Wang, Pengjun Wang, Zenglan Xing, Shichao |
author_sort | Liu, Xiu |
collection | PubMed |
description | Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target. |
format | Online Article Text |
id | pubmed-7061907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70619072020-03-17 The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia Liu, Xiu Lv, Qiulan Ren, Hongyan Gao, Liu Zhao, Peng Yang, Xiaomin Yang, Guanpin Xu, Daxing Wang, Guangtao Yang, Wan Wang, Pengjun Wang, Zenglan Xing, Shichao PeerJ Microbiology Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target. PeerJ Inc. 2020-03-06 /pmc/articles/PMC7061907/ /pubmed/32185104 http://dx.doi.org/10.7717/peerj.8664 Text en ©2020 Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Microbiology Liu, Xiu Lv, Qiulan Ren, Hongyan Gao, Liu Zhao, Peng Yang, Xiaomin Yang, Guanpin Xu, Daxing Wang, Guangtao Yang, Wan Wang, Pengjun Wang, Zenglan Xing, Shichao The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title_full | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title_fullStr | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title_full_unstemmed | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title_short | The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
title_sort | altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061907/ https://www.ncbi.nlm.nih.gov/pubmed/32185104 http://dx.doi.org/10.7717/peerj.8664 |
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