Cargando…

The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia

Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the pot...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiu, Lv, Qiulan, Ren, Hongyan, Gao, Liu, Zhao, Peng, Yang, Xiaomin, Yang, Guanpin, Xu, Daxing, Wang, Guangtao, Yang, Wan, Wang, Pengjun, Wang, Zenglan, Xing, Shichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061907/
https://www.ncbi.nlm.nih.gov/pubmed/32185104
http://dx.doi.org/10.7717/peerj.8664
_version_ 1783504462617247744
author Liu, Xiu
Lv, Qiulan
Ren, Hongyan
Gao, Liu
Zhao, Peng
Yang, Xiaomin
Yang, Guanpin
Xu, Daxing
Wang, Guangtao
Yang, Wan
Wang, Pengjun
Wang, Zenglan
Xing, Shichao
author_facet Liu, Xiu
Lv, Qiulan
Ren, Hongyan
Gao, Liu
Zhao, Peng
Yang, Xiaomin
Yang, Guanpin
Xu, Daxing
Wang, Guangtao
Yang, Wan
Wang, Pengjun
Wang, Zenglan
Xing, Shichao
author_sort Liu, Xiu
collection PubMed
description Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target.
format Online
Article
Text
id pubmed-7061907
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-70619072020-03-17 The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia Liu, Xiu Lv, Qiulan Ren, Hongyan Gao, Liu Zhao, Peng Yang, Xiaomin Yang, Guanpin Xu, Daxing Wang, Guangtao Yang, Wan Wang, Pengjun Wang, Zenglan Xing, Shichao PeerJ Microbiology Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target. PeerJ Inc. 2020-03-06 /pmc/articles/PMC7061907/ /pubmed/32185104 http://dx.doi.org/10.7717/peerj.8664 Text en ©2020 Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Liu, Xiu
Lv, Qiulan
Ren, Hongyan
Gao, Liu
Zhao, Peng
Yang, Xiaomin
Yang, Guanpin
Xu, Daxing
Wang, Guangtao
Yang, Wan
Wang, Pengjun
Wang, Zenglan
Xing, Shichao
The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title_full The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title_fullStr The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title_full_unstemmed The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title_short The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
title_sort altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061907/
https://www.ncbi.nlm.nih.gov/pubmed/32185104
http://dx.doi.org/10.7717/peerj.8664
work_keys_str_mv AT liuxiu thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT lvqiulan thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT renhongyan thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT gaoliu thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT zhaopeng thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangxiaomin thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangguanpin thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT xudaxing thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangguangtao thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangwan thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangpengjun thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangzenglan thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT xingshichao thealteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT liuxiu alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT lvqiulan alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT renhongyan alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT gaoliu alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT zhaopeng alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangxiaomin alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangguanpin alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT xudaxing alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangguangtao alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT yangwan alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangpengjun alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT wangzenglan alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia
AT xingshichao alteredgutmicrobiotaofhighpurineinducedhyperuricemiaratsanditscorrelationwithhyperuricemia