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Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress

Silicon (Si), the second most abundant element on earth, remains unavailable for plants' uptake due to its poor solubility. Microbial interventions to convert it in soluble forms are well documented. However, studies on discrimination of Si and P solubilizing microbes due to common estimation m...

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Autores principales: Bist, Vidisha, Niranjan, Abhishek, Ranjan, Manish, Lehri, Alok, Seem, Karishma, Srivastava, Suchi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061934/
https://www.ncbi.nlm.nih.gov/pubmed/32194577
http://dx.doi.org/10.3389/fpls.2020.00028
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author Bist, Vidisha
Niranjan, Abhishek
Ranjan, Manish
Lehri, Alok
Seem, Karishma
Srivastava, Suchi
author_facet Bist, Vidisha
Niranjan, Abhishek
Ranjan, Manish
Lehri, Alok
Seem, Karishma
Srivastava, Suchi
author_sort Bist, Vidisha
collection PubMed
description Silicon (Si), the second most abundant element on earth, remains unavailable for plants' uptake due to its poor solubility. Microbial interventions to convert it in soluble forms are well documented. However, studies on discrimination of Si and P solubilizing microbes due to common estimation method and sharing of solubilization mechanism are still obscure. A defined differential media, i.e. silicon-solubilizing media (NBRISSM) is developed to screen Si solubilizers. NBRISN13 (Bacillus amyloliquefaciens), a Si solubilizer, exhibiting antagonistic property against Rhizoctonia solani, was further validated for disease resistance. The key finding of the work is that NBRISSM is a novel differential media for screening Si solubilizers, distinct from P solubilizers. Dominance of Pseudomonas and Bacillus spp. for the function of Si solubilization was observed during diversity analysis of Si solubilizers isolated from different rhizospheres. Sphingobacterium sp., a different strain has been identified for silicon solubilization other than Pseudomonas and Bacillus sp. Role of acidic phosphatase during Si solubilization has been firstly reported in our study in addition to other pH dependent phenomenon. Study also showed the combinatorial effect of feldspar and NBRISN13 on elicited immune response through (i) increased Si uptake, (ii) reduced disease severity, (iii) modulation of cell wall degrading and antioxidative enzyme activities, and (iv) induced defense responsive gene expression.
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spelling pubmed-70619342020-03-19 Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress Bist, Vidisha Niranjan, Abhishek Ranjan, Manish Lehri, Alok Seem, Karishma Srivastava, Suchi Front Plant Sci Plant Science Silicon (Si), the second most abundant element on earth, remains unavailable for plants' uptake due to its poor solubility. Microbial interventions to convert it in soluble forms are well documented. However, studies on discrimination of Si and P solubilizing microbes due to common estimation method and sharing of solubilization mechanism are still obscure. A defined differential media, i.e. silicon-solubilizing media (NBRISSM) is developed to screen Si solubilizers. NBRISN13 (Bacillus amyloliquefaciens), a Si solubilizer, exhibiting antagonistic property against Rhizoctonia solani, was further validated for disease resistance. The key finding of the work is that NBRISSM is a novel differential media for screening Si solubilizers, distinct from P solubilizers. Dominance of Pseudomonas and Bacillus spp. for the function of Si solubilization was observed during diversity analysis of Si solubilizers isolated from different rhizospheres. Sphingobacterium sp., a different strain has been identified for silicon solubilization other than Pseudomonas and Bacillus sp. Role of acidic phosphatase during Si solubilization has been firstly reported in our study in addition to other pH dependent phenomenon. Study also showed the combinatorial effect of feldspar and NBRISN13 on elicited immune response through (i) increased Si uptake, (ii) reduced disease severity, (iii) modulation of cell wall degrading and antioxidative enzyme activities, and (iv) induced defense responsive gene expression. Frontiers Media S.A. 2020-02-28 /pmc/articles/PMC7061934/ /pubmed/32194577 http://dx.doi.org/10.3389/fpls.2020.00028 Text en Copyright © 2020 Bist, Niranjan, Ranjan, Lehri, Seem and Srivastava http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bist, Vidisha
Niranjan, Abhishek
Ranjan, Manish
Lehri, Alok
Seem, Karishma
Srivastava, Suchi
Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title_full Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title_fullStr Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title_full_unstemmed Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title_short Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress
title_sort silicon-solubilizing media and its implication for characterization of bacteria to mitigate biotic stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061934/
https://www.ncbi.nlm.nih.gov/pubmed/32194577
http://dx.doi.org/10.3389/fpls.2020.00028
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