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From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response

Glioblastomas are aggressive primary brain tumors known for their inter- and intratumor heterogeneity. This disease is uniformly fatal, with intratumor heterogeneity the major reason for treatment failure and recurrence. Just like the nature vs nurture debate, heterogeneity can arise from intrinsic...

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Autores principales: Gallaher, Jill A., Massey, Susan C., Hawkins-Daarud, Andrea, Noticewala, Sonal S., Rockne, Russell C., Johnston, Sandra K., Gonzalez-Cuyar, Luis, Juliano, Joseph, Gil, Orlando, Swanson, Kristin R., Canoll, Peter, Anderson, Alexander R. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062288/
https://www.ncbi.nlm.nih.gov/pubmed/32101537
http://dx.doi.org/10.1371/journal.pcbi.1007672
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author Gallaher, Jill A.
Massey, Susan C.
Hawkins-Daarud, Andrea
Noticewala, Sonal S.
Rockne, Russell C.
Johnston, Sandra K.
Gonzalez-Cuyar, Luis
Juliano, Joseph
Gil, Orlando
Swanson, Kristin R.
Canoll, Peter
Anderson, Alexander R. A.
author_facet Gallaher, Jill A.
Massey, Susan C.
Hawkins-Daarud, Andrea
Noticewala, Sonal S.
Rockne, Russell C.
Johnston, Sandra K.
Gonzalez-Cuyar, Luis
Juliano, Joseph
Gil, Orlando
Swanson, Kristin R.
Canoll, Peter
Anderson, Alexander R. A.
author_sort Gallaher, Jill A.
collection PubMed
description Glioblastomas are aggressive primary brain tumors known for their inter- and intratumor heterogeneity. This disease is uniformly fatal, with intratumor heterogeneity the major reason for treatment failure and recurrence. Just like the nature vs nurture debate, heterogeneity can arise from intrinsic or environmental influences. Whilst it is impossible to clinically separate observed behavior of cells from their environmental context, using a mathematical framework combined with multiscale data gives us insight into the relative roles of variation from different sources. To better understand the implications of intratumor heterogeneity on therapeutic outcomes, we created a hybrid agent-based mathematical model that captures both the overall tumor kinetics and the individual cellular behavior. We track single cells as agents, cell density on a coarser scale, and growth factor diffusion and dynamics on a finer scale over time and space. Our model parameters were fit utilizing serial MRI imaging and cell tracking data from ex vivo tissue slices acquired from a growth-factor driven glioblastoma murine model. When fitting our model to serial imaging only, there was a spectrum of equally-good parameter fits corresponding to a wide range of phenotypic behaviors. When fitting our model using imaging and cell scale data, we determined that environmental heterogeneity alone is insufficient to match the single cell data, and intrinsic heterogeneity is required to fully capture the migration behavior. The wide spectrum of in silico tumors also had a wide variety of responses to an application of an anti-proliferative treatment. Recurrent tumors were generally less proliferative than pre-treatment tumors as measured via the model simulations and validated from human GBM patient histology. Further, we found that all tumors continued to grow with an anti-migratory treatment alone, but the anti-proliferative/anti-migratory combination generally showed improvement over an anti-proliferative treatment alone. Together our results emphasize the need to better understand the underlying phenotypes and tumor heterogeneity present in a tumor when designing therapeutic regimens.
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spelling pubmed-70622882020-03-23 From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response Gallaher, Jill A. Massey, Susan C. Hawkins-Daarud, Andrea Noticewala, Sonal S. Rockne, Russell C. Johnston, Sandra K. Gonzalez-Cuyar, Luis Juliano, Joseph Gil, Orlando Swanson, Kristin R. Canoll, Peter Anderson, Alexander R. A. PLoS Comput Biol Research Article Glioblastomas are aggressive primary brain tumors known for their inter- and intratumor heterogeneity. This disease is uniformly fatal, with intratumor heterogeneity the major reason for treatment failure and recurrence. Just like the nature vs nurture debate, heterogeneity can arise from intrinsic or environmental influences. Whilst it is impossible to clinically separate observed behavior of cells from their environmental context, using a mathematical framework combined with multiscale data gives us insight into the relative roles of variation from different sources. To better understand the implications of intratumor heterogeneity on therapeutic outcomes, we created a hybrid agent-based mathematical model that captures both the overall tumor kinetics and the individual cellular behavior. We track single cells as agents, cell density on a coarser scale, and growth factor diffusion and dynamics on a finer scale over time and space. Our model parameters were fit utilizing serial MRI imaging and cell tracking data from ex vivo tissue slices acquired from a growth-factor driven glioblastoma murine model. When fitting our model to serial imaging only, there was a spectrum of equally-good parameter fits corresponding to a wide range of phenotypic behaviors. When fitting our model using imaging and cell scale data, we determined that environmental heterogeneity alone is insufficient to match the single cell data, and intrinsic heterogeneity is required to fully capture the migration behavior. The wide spectrum of in silico tumors also had a wide variety of responses to an application of an anti-proliferative treatment. Recurrent tumors were generally less proliferative than pre-treatment tumors as measured via the model simulations and validated from human GBM patient histology. Further, we found that all tumors continued to grow with an anti-migratory treatment alone, but the anti-proliferative/anti-migratory combination generally showed improvement over an anti-proliferative treatment alone. Together our results emphasize the need to better understand the underlying phenotypes and tumor heterogeneity present in a tumor when designing therapeutic regimens. Public Library of Science 2020-02-26 /pmc/articles/PMC7062288/ /pubmed/32101537 http://dx.doi.org/10.1371/journal.pcbi.1007672 Text en © 2020 Gallaher et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gallaher, Jill A.
Massey, Susan C.
Hawkins-Daarud, Andrea
Noticewala, Sonal S.
Rockne, Russell C.
Johnston, Sandra K.
Gonzalez-Cuyar, Luis
Juliano, Joseph
Gil, Orlando
Swanson, Kristin R.
Canoll, Peter
Anderson, Alexander R. A.
From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title_full From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title_fullStr From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title_full_unstemmed From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title_short From cells to tissue: How cell scale heterogeneity impacts glioblastoma growth and treatment response
title_sort from cells to tissue: how cell scale heterogeneity impacts glioblastoma growth and treatment response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062288/
https://www.ncbi.nlm.nih.gov/pubmed/32101537
http://dx.doi.org/10.1371/journal.pcbi.1007672
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