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Dynamics of human monocytes and airway macrophages during healthy aging and after transplant

The ontogeny of airway macrophages (AMs) in human lung and their contribution to disease are poorly mapped out. In mice, aging is associated with an increasing proportion of peripherally, as opposed to perinatally derived AMs. We sought to understand AM ontogeny in human lung during healthy aging an...

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Autores principales: Byrne, Adam J., Powell, Joseph E., O’Sullivan, Brendan J., Ogger, Patricia P., Hoffland, Ashley, Cook, James, Bonner, Katie L., Hewitt, Richard J., Wolf, Simone, Ghai, Poonam, Walker, Simone A., Lukowski, Samuel W., Molyneaux, Philip L., Saglani, Sejal, Chambers, Daniel C., Maher, Toby M., Lloyd, Clare M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062517/
https://www.ncbi.nlm.nih.gov/pubmed/31917836
http://dx.doi.org/10.1084/jem.20191236
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author Byrne, Adam J.
Powell, Joseph E.
O’Sullivan, Brendan J.
Ogger, Patricia P.
Hoffland, Ashley
Cook, James
Bonner, Katie L.
Hewitt, Richard J.
Wolf, Simone
Ghai, Poonam
Walker, Simone A.
Lukowski, Samuel W.
Molyneaux, Philip L.
Saglani, Sejal
Chambers, Daniel C.
Maher, Toby M.
Lloyd, Clare M.
author_facet Byrne, Adam J.
Powell, Joseph E.
O’Sullivan, Brendan J.
Ogger, Patricia P.
Hoffland, Ashley
Cook, James
Bonner, Katie L.
Hewitt, Richard J.
Wolf, Simone
Ghai, Poonam
Walker, Simone A.
Lukowski, Samuel W.
Molyneaux, Philip L.
Saglani, Sejal
Chambers, Daniel C.
Maher, Toby M.
Lloyd, Clare M.
author_sort Byrne, Adam J.
collection PubMed
description The ontogeny of airway macrophages (AMs) in human lung and their contribution to disease are poorly mapped out. In mice, aging is associated with an increasing proportion of peripherally, as opposed to perinatally derived AMs. We sought to understand AM ontogeny in human lung during healthy aging and after transplant. We characterized monocyte/macrophage populations from the peripheral blood and airways of healthy volunteers across infancy/childhood (2–12 yr), maturity (20–50 yr), and older adulthood (>50 yr). Single-cell RNA sequencing (scRNA-seq) was performed on airway inflammatory cells isolated from sex-mismatched lung transplant recipients. During healthy aging, the proportions of blood and bronchoalveolar lavage (BAL) classical monocytes peak in adulthood and decline in older adults. scRNA-seq of BAL cells from lung transplant recipients indicates that after transplant, the majority of AMs are recipient derived. These data show that during aging, the peripheral monocyte phenotype is consistent with that found in the airways and, furthermore, that the majority of human AMs after transplant are derived from circulating monocytes.
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spelling pubmed-70625172020-03-17 Dynamics of human monocytes and airway macrophages during healthy aging and after transplant Byrne, Adam J. Powell, Joseph E. O’Sullivan, Brendan J. Ogger, Patricia P. Hoffland, Ashley Cook, James Bonner, Katie L. Hewitt, Richard J. Wolf, Simone Ghai, Poonam Walker, Simone A. Lukowski, Samuel W. Molyneaux, Philip L. Saglani, Sejal Chambers, Daniel C. Maher, Toby M. Lloyd, Clare M. J Exp Med Brief Definitive Report The ontogeny of airway macrophages (AMs) in human lung and their contribution to disease are poorly mapped out. In mice, aging is associated with an increasing proportion of peripherally, as opposed to perinatally derived AMs. We sought to understand AM ontogeny in human lung during healthy aging and after transplant. We characterized monocyte/macrophage populations from the peripheral blood and airways of healthy volunteers across infancy/childhood (2–12 yr), maturity (20–50 yr), and older adulthood (>50 yr). Single-cell RNA sequencing (scRNA-seq) was performed on airway inflammatory cells isolated from sex-mismatched lung transplant recipients. During healthy aging, the proportions of blood and bronchoalveolar lavage (BAL) classical monocytes peak in adulthood and decline in older adults. scRNA-seq of BAL cells from lung transplant recipients indicates that after transplant, the majority of AMs are recipient derived. These data show that during aging, the peripheral monocyte phenotype is consistent with that found in the airways and, furthermore, that the majority of human AMs after transplant are derived from circulating monocytes. Rockefeller University Press 2020-01-09 /pmc/articles/PMC7062517/ /pubmed/31917836 http://dx.doi.org/10.1084/jem.20191236 Text en © 2020 Byrne et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Definitive Report
Byrne, Adam J.
Powell, Joseph E.
O’Sullivan, Brendan J.
Ogger, Patricia P.
Hoffland, Ashley
Cook, James
Bonner, Katie L.
Hewitt, Richard J.
Wolf, Simone
Ghai, Poonam
Walker, Simone A.
Lukowski, Samuel W.
Molyneaux, Philip L.
Saglani, Sejal
Chambers, Daniel C.
Maher, Toby M.
Lloyd, Clare M.
Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title_full Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title_fullStr Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title_full_unstemmed Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title_short Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
title_sort dynamics of human monocytes and airway macrophages during healthy aging and after transplant
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062517/
https://www.ncbi.nlm.nih.gov/pubmed/31917836
http://dx.doi.org/10.1084/jem.20191236
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