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In vitro Models of Breast Cancer Metastatic Dormancy

Delayed relapses at distant sites are a common clinical observation for certain types of cancers after removal of primary tumor, such as breast and prostate cancer. This evidence has been explained by postulating a long period during which disseminated cancer cells (DCCs) survive in a foreign enviro...

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Autores principales: Montagner, Marco, Sahai, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062644/
https://www.ncbi.nlm.nih.gov/pubmed/32195244
http://dx.doi.org/10.3389/fcell.2020.00037
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author Montagner, Marco
Sahai, Erik
author_facet Montagner, Marco
Sahai, Erik
author_sort Montagner, Marco
collection PubMed
description Delayed relapses at distant sites are a common clinical observation for certain types of cancers after removal of primary tumor, such as breast and prostate cancer. This evidence has been explained by postulating a long period during which disseminated cancer cells (DCCs) survive in a foreign environment without developing into overt metastasis. Because of the asymptomatic nature of this phenomenon, isolation, and analysis of disseminated dormant cancer cells from clinically disease-free patients is ethically and technically highly problematic and currently these data are largely limited to the bone marrow. That said, detecting, profiling and treating indolent metastatic lesions before the onset of relapse is the imperative. To overcome this major limitation many laboratories developed in vitro models of the metastatic niche for different organs and different types of cancers. In this review we focus specifically on in vitro models designed to study metastatic dormancy of breast cancer cells (BCCs). We provide an overview of the BCCs employed in the different organotypic systems and address the components of the metastatic microenvironment that have been shown to impact on the dormant phenotype: tissue architecture, stromal cells, biochemical environment, oxygen levels, cell density. A brief description of the organ-specific in vitro models for bone, liver, and lung is provided. Finally, we discuss the strategies employed so far for the validation of the different systems.
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spelling pubmed-70626442020-03-19 In vitro Models of Breast Cancer Metastatic Dormancy Montagner, Marco Sahai, Erik Front Cell Dev Biol Cell and Developmental Biology Delayed relapses at distant sites are a common clinical observation for certain types of cancers after removal of primary tumor, such as breast and prostate cancer. This evidence has been explained by postulating a long period during which disseminated cancer cells (DCCs) survive in a foreign environment without developing into overt metastasis. Because of the asymptomatic nature of this phenomenon, isolation, and analysis of disseminated dormant cancer cells from clinically disease-free patients is ethically and technically highly problematic and currently these data are largely limited to the bone marrow. That said, detecting, profiling and treating indolent metastatic lesions before the onset of relapse is the imperative. To overcome this major limitation many laboratories developed in vitro models of the metastatic niche for different organs and different types of cancers. In this review we focus specifically on in vitro models designed to study metastatic dormancy of breast cancer cells (BCCs). We provide an overview of the BCCs employed in the different organotypic systems and address the components of the metastatic microenvironment that have been shown to impact on the dormant phenotype: tissue architecture, stromal cells, biochemical environment, oxygen levels, cell density. A brief description of the organ-specific in vitro models for bone, liver, and lung is provided. Finally, we discuss the strategies employed so far for the validation of the different systems. Frontiers Media S.A. 2020-03-03 /pmc/articles/PMC7062644/ /pubmed/32195244 http://dx.doi.org/10.3389/fcell.2020.00037 Text en Copyright © 2020 Montagner and Sahai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Montagner, Marco
Sahai, Erik
In vitro Models of Breast Cancer Metastatic Dormancy
title In vitro Models of Breast Cancer Metastatic Dormancy
title_full In vitro Models of Breast Cancer Metastatic Dormancy
title_fullStr In vitro Models of Breast Cancer Metastatic Dormancy
title_full_unstemmed In vitro Models of Breast Cancer Metastatic Dormancy
title_short In vitro Models of Breast Cancer Metastatic Dormancy
title_sort in vitro models of breast cancer metastatic dormancy
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062644/
https://www.ncbi.nlm.nih.gov/pubmed/32195244
http://dx.doi.org/10.3389/fcell.2020.00037
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