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Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening
Red wolfberry (or goji berry, Lycium barbarum; LB) is an important agricultural product with a high content of pharmacologically important secondary metabolites such as phenylpropanoids. A close relative, black wolfberry (L. ruthenicum; LR), endemic to the salinized deserts of northwestern China, is...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062791/ https://www.ncbi.nlm.nih.gov/pubmed/32152358 http://dx.doi.org/10.1038/s41598-020-61064-5 |
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author | Zhao, Jianhua Li, Haoxia Yin, Yue An, Wei Qin, Xiaoya Wang, Yajun Li, Yanlong Fan, Yunfang Cao, Youlong |
author_facet | Zhao, Jianhua Li, Haoxia Yin, Yue An, Wei Qin, Xiaoya Wang, Yajun Li, Yanlong Fan, Yunfang Cao, Youlong |
author_sort | Zhao, Jianhua |
collection | PubMed |
description | Red wolfberry (or goji berry, Lycium barbarum; LB) is an important agricultural product with a high content of pharmacologically important secondary metabolites such as phenylpropanoids. A close relative, black wolfberry (L. ruthenicum; LR), endemic to the salinized deserts of northwestern China, is used only locally. The two fruits exhibit many morphological and phytochemical differences, but genetic mechanisms underlying them remain poorly explored. In order to identify the genes of interest for further studies, we studied transcriptomic (Illumina HiSeq) and metabolomic (LC-MS) profiles of the two fruits during five developmental stages (young to ripe). As expected, we identified much higher numbers of significantly differentially regulated genes (DEGs) than metabolites. The highest numbers were identified in pairwise comparisons including the first stage for both species, but total numbers were consistently somewhat lower for the LR. The number of differentially regulated metabolites in pairwise comparisons of developmental stages varied from 66 (stages 3 vs 4) to 133 (stages 2 vs 5) in both species. We identified a number of genes (e.g. AAT1, metE, pip) and metabolites (e.g. rutin, raffinose, galactinol, trehalose, citrulline and DL-arginine) that may be of interest to future functional studies of stress adaptation in plants. As LB is also highly suitable for combating soil desertification and alleviating soil salinity/alkalinity/pollution, its potential for human use may be much wider than its current, highly localized, relevance. |
format | Online Article Text |
id | pubmed-7062791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70627912020-03-18 Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening Zhao, Jianhua Li, Haoxia Yin, Yue An, Wei Qin, Xiaoya Wang, Yajun Li, Yanlong Fan, Yunfang Cao, Youlong Sci Rep Article Red wolfberry (or goji berry, Lycium barbarum; LB) is an important agricultural product with a high content of pharmacologically important secondary metabolites such as phenylpropanoids. A close relative, black wolfberry (L. ruthenicum; LR), endemic to the salinized deserts of northwestern China, is used only locally. The two fruits exhibit many morphological and phytochemical differences, but genetic mechanisms underlying them remain poorly explored. In order to identify the genes of interest for further studies, we studied transcriptomic (Illumina HiSeq) and metabolomic (LC-MS) profiles of the two fruits during five developmental stages (young to ripe). As expected, we identified much higher numbers of significantly differentially regulated genes (DEGs) than metabolites. The highest numbers were identified in pairwise comparisons including the first stage for both species, but total numbers were consistently somewhat lower for the LR. The number of differentially regulated metabolites in pairwise comparisons of developmental stages varied from 66 (stages 3 vs 4) to 133 (stages 2 vs 5) in both species. We identified a number of genes (e.g. AAT1, metE, pip) and metabolites (e.g. rutin, raffinose, galactinol, trehalose, citrulline and DL-arginine) that may be of interest to future functional studies of stress adaptation in plants. As LB is also highly suitable for combating soil desertification and alleviating soil salinity/alkalinity/pollution, its potential for human use may be much wider than its current, highly localized, relevance. Nature Publishing Group UK 2020-03-09 /pmc/articles/PMC7062791/ /pubmed/32152358 http://dx.doi.org/10.1038/s41598-020-61064-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhao, Jianhua Li, Haoxia Yin, Yue An, Wei Qin, Xiaoya Wang, Yajun Li, Yanlong Fan, Yunfang Cao, Youlong Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title | Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title_full | Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title_fullStr | Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title_full_unstemmed | Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title_short | Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening |
title_sort | transcriptomic and metabolomic analyses of lycium ruthenicum and lycium barbarum fruits during ripening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062791/ https://www.ncbi.nlm.nih.gov/pubmed/32152358 http://dx.doi.org/10.1038/s41598-020-61064-5 |
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