Cargando…

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

Antimicrobial resistance (AMR) is a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS) is a powerful tool to monitor AMR determinants. However, plasmids are difficult to reconstruct from WGS data. This study aimed to improve th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Berbers, Bas, Saltykova, Assia, Garcia-Graells, Cristina, Philipp, Patrick, Arella, Fabrice, Marchal, Kathleen, Winand, Raf, Vanneste, Kevin, Roosens, Nancy H. C., De Keersmaecker, Sigrid C. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062872/ https://www.ncbi.nlm.nih.gov/pubmed/32152350 http://dx.doi.org/10.1038/s41598-020-61158-0

Ejemplares similares

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO
por: Fraiture, Marie-Alice, et al.
Publicado: (2018)

Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
por: Fraiture, Marie-Alice, et al.
Publicado: (2020)

Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
por: Saltykova, Assia, et al.
Publicado: (2022)

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
por: Buytaers, Florence E., et al.
Publicado: (2021)

Using a combination of short- and long-read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum beta-lactamases (ESBLs) in clinical Shigella and Salmonella isolates in Belgium
por: Berbers, Bas, et al.
Publicado: (2023)

MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
por: Fraiture, Marie-Alice, et al.
Publicado: (2019)

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination
por: Berbers, Bas, et al.
Publicado: (2020)

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:-
por: Saltykova, Assia, et al.
Publicado: (2018)

Evaluation of WGS performance for bacterial pathogen characterization with the Illumina technology optimized for time-critical situations
por: Bogaerts, Bert, et al.
Publicado: (2021)

Detailed Evaluation of Data Analysis Tools for Subtyping of Bacterial Isolates Based on Whole Genome Sequencing: Neisseria meningitidis as a Proof of Concept
por: Saltykova, Assia, et al.
Publicado: (2019)

Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin
por: Barbau-piednoir, Elodie, et al.
Publicado: (2015)

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine
por: Buytaers, Florence E., et al.
Publicado: (2020)

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies
por: Buytaers, Florence E., et al.
Publicado: (2021)

Phylogenomic Investigation of Increasing Fluoroquinolone Resistance among Belgian Cases of Shigellosis between 2013 and 2018 Indicates Both Travel-Related Imports and Domestic Circulation
por: Bogaerts, Bert, et al.
Publicado: (2021)

Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java
por: Gand, Mathieu, et al.
Publicado: (2019)

Genome Sequence of EU-Unauthorized Genetically Modified Bacillus subtilis Strain 2014-3557 Overproducing Riboflavin, Isolated from a Vitamin B2 80% Feed Additive
por: Barbau-Piednoir, Elodie, et al.
Publicado: (2015)

Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study
por: Saltykova, Assia, et al.
Publicado: (2020)

Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak
por: Buytaers, Florence E., et al.
Publicado: (2021)

A Bioinformatics Whole-Genome Sequencing Workflow for Clinical Mycobacterium tuberculosis Complex Isolate Analysis, Validated Using a Reference Collection Extensively Characterized with Conventional Methods and In Silico Approaches
por: Bogaerts, Bert, et al.
Publicado: (2021)

Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
por: Bogaerts, Bert, et al.
Publicado: (2021)

Targeting the 16S rRNA Gene for Bacterial Identification in Complex Mixed Samples: Comparative Evaluation of Second (Illumina) and Third (Oxford Nanopore Technologies) Generation Sequencing Technologies
por: Winand, Raf, et al.
Publicado: (2019)

Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: Neisseria meningitidis as a Proof-of-Concept
por: Bogaerts, Bert, et al.
Publicado: (2019)

Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and antimicrobial resistance surveillance
por: Bloemen, Bram, et al.
Publicado: (2023)

Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products
por: D’aes, Jolien, et al.
Publicado: (2022)

Unauthorized Version
Publicado: (1991)

Unauthorized Visitors
Publicado: (1876)

Whole-genome-based phylogenomic analysis of the Belgian 2016–2017 influenza A(H3N2) outbreak season allows improved surveillance
por: Van Poelvoorde, Laura A. E., et al.
Publicado: (2021)

Characterization of Genetically Modified Microorganisms Using Short- and Long-Read Whole-Genome Sequencing Reveals Contaminations of Related Origin in Multiple Commercial Food Enzyme Products
por: D’aes, Jolien, et al.
Publicado: (2021)

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study
por: Libert, Xavier, et al.
Publicado: (2017)

First detection of a plasmid-encoded New-Delhi metallo-beta-lactamase-1 (NDM-1) producing Acinetobacter baumannii using whole genome sequencing, isolated in a clinical setting in Benin
por: Yehouenou, Carine, et al.
Publicado: (2021)

Unauthorized Renewal of Prescriptions
Publicado: (1869)

The unauthorized version : truth and fiction in the Bible
por: Lane Fox, Robin, 1946-
Publicado: (1993)

Unauthorized Use of M.D.
Publicado: (1875)

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
por: Nouws, Stéphanie, et al.
Publicado: (2020)

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks
por: Nouws, Stéphanie, et al.
Publicado: (2021)

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria
por: Gand, Mathieu, et al.
Publicado: (2023)

Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B(2) products sold on the Belgian market using whole-genome sequencing
por: Bogaerts, Bert, et al.
Publicado: (2023)

Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array
por: Wuyts, Véronique, et al.
Publicado: (2015)

Metagenomics to Detect and Characterize Viruses in Food Samples at Genome Level? Lessons Learnt from a Norovirus Study
por: Buytaers, Florence E., et al.
Publicado: (2022)

Transforming Shiga toxin-producing Escherichia coli surveillance through whole genome sequencing in food safety practices
por: Nouws, Stéphanie, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_98pt1f4htd5hpr9t0742m54vgi