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Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array
Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challen...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062878/ https://www.ncbi.nlm.nih.gov/pubmed/32152369 http://dx.doi.org/10.1038/s41598-020-61325-3 |
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author | Yoo, Jisoo Kwak, Hankyul Kwon, Juyoung Ha, Go Eun Lee, Elliot H. Song, Seungwoo Na, Jukwan Lee, Hyo-Jung Lee, Jaejun Hwangbo, Areum Cha, Eunkyung Chae, Youngcheol Cheong, Eunji Choi, Heon-Jin |
author_facet | Yoo, Jisoo Kwak, Hankyul Kwon, Juyoung Ha, Go Eun Lee, Elliot H. Song, Seungwoo Na, Jukwan Lee, Hyo-Jung Lee, Jaejun Hwangbo, Areum Cha, Eunkyung Chae, Youngcheol Cheong, Eunji Choi, Heon-Jin |
author_sort | Yoo, Jisoo |
collection | PubMed |
description | Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challenges are still remained to conquer this important approach. Here, we demonstrate long-term intracellular recording by combining a vertical nanowire multi electrode array (VNMEA) with optogenetic stimulation to minimally disrupt cell survival and functions during intracellular access and measurement. We synthesized small-diameter and high-aspect-ratio silicon nanowires to spontaneously penetrate into single cells, and used light to modulate the cell’s responsiveness. The light-induced intra- and extracellular activities of individual optogenetically-modified cells were measured simultaneously, and each cell showed distinctly different measurement characteristics according to the cell-electrode configuration. Intracellular recordings were achieved continuously and reliably without signal interference and attenuation over 24 hours. The integration of two controllable techniques, vertically grown nanowire electrodes and optogenetics, expands the strategies for discovering the mechanisms for crucial physiological and dynamic processes in various types of cells. |
format | Online Article Text |
id | pubmed-7062878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70628782020-03-18 Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array Yoo, Jisoo Kwak, Hankyul Kwon, Juyoung Ha, Go Eun Lee, Elliot H. Song, Seungwoo Na, Jukwan Lee, Hyo-Jung Lee, Jaejun Hwangbo, Areum Cha, Eunkyung Chae, Youngcheol Cheong, Eunji Choi, Heon-Jin Sci Rep Article Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challenges are still remained to conquer this important approach. Here, we demonstrate long-term intracellular recording by combining a vertical nanowire multi electrode array (VNMEA) with optogenetic stimulation to minimally disrupt cell survival and functions during intracellular access and measurement. We synthesized small-diameter and high-aspect-ratio silicon nanowires to spontaneously penetrate into single cells, and used light to modulate the cell’s responsiveness. The light-induced intra- and extracellular activities of individual optogenetically-modified cells were measured simultaneously, and each cell showed distinctly different measurement characteristics according to the cell-electrode configuration. Intracellular recordings were achieved continuously and reliably without signal interference and attenuation over 24 hours. The integration of two controllable techniques, vertically grown nanowire electrodes and optogenetics, expands the strategies for discovering the mechanisms for crucial physiological and dynamic processes in various types of cells. Nature Publishing Group UK 2020-03-09 /pmc/articles/PMC7062878/ /pubmed/32152369 http://dx.doi.org/10.1038/s41598-020-61325-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yoo, Jisoo Kwak, Hankyul Kwon, Juyoung Ha, Go Eun Lee, Elliot H. Song, Seungwoo Na, Jukwan Lee, Hyo-Jung Lee, Jaejun Hwangbo, Areum Cha, Eunkyung Chae, Youngcheol Cheong, Eunji Choi, Heon-Jin Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title | Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title_full | Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title_fullStr | Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title_full_unstemmed | Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title_short | Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array |
title_sort | long-term intracellular recording of optogenetically-induced electrical activities using vertical nanowire multi electrode array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062878/ https://www.ncbi.nlm.nih.gov/pubmed/32152369 http://dx.doi.org/10.1038/s41598-020-61325-3 |
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