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Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying

Propionibacterium freudenreichii is a beneficial bacterium widely used in food as a probiotic and as a cheese-ripening starter. In these different applications, it is produced, dried, and stored before being used. Both freeze-drying and spray-drying were considered for this purpose. Freeze-drying is...

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Autores principales: Gaucher, Floriane, Rabah, Houem, Kponouglo, Koffigan, Bonnassie, Sylvie, Pottier, Sandrine, Dolivet, Anne, Marchand, Pierre, Jeantet, Romain, Blanc, Philippe, Jan, Gwénaël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062905/
https://www.ncbi.nlm.nih.gov/pubmed/32076782
http://dx.doi.org/10.1007/s00253-020-10425-1
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author Gaucher, Floriane
Rabah, Houem
Kponouglo, Koffigan
Bonnassie, Sylvie
Pottier, Sandrine
Dolivet, Anne
Marchand, Pierre
Jeantet, Romain
Blanc, Philippe
Jan, Gwénaël
author_facet Gaucher, Floriane
Rabah, Houem
Kponouglo, Koffigan
Bonnassie, Sylvie
Pottier, Sandrine
Dolivet, Anne
Marchand, Pierre
Jeantet, Romain
Blanc, Philippe
Jan, Gwénaël
author_sort Gaucher, Floriane
collection PubMed
description Propionibacterium freudenreichii is a beneficial bacterium widely used in food as a probiotic and as a cheese-ripening starter. In these different applications, it is produced, dried, and stored before being used. Both freeze-drying and spray-drying were considered for this purpose. Freeze-drying is a discontinuous process that is energy-consuming but that allows high cell survival. Spray-drying is a continuous process that is more energy-efficient but that can lead to massive bacterial death related to heat, osmotic, and oxidative stresses. We have shown that P. freudenreichii cultivated in hyperconcentrated rich media can be spray-dried with limited bacterial death. However, the general stress tolerance conferred by this hyperosmotic constraint remained a black box. In this study, we modulated P. freudenreichii growth conditions and monitored both osmoprotectant accumulation and stress tolerance acquisition. Changing the ratio between the carbohydrates provided and non-protein nitrogen during growth under osmotic constraint modulated osmoprotectant accumulation. This, in turn, was correlated with P. freudenreichii tolerance towards different stresses, on the one hand, and towards freeze-drying and spray-drying, on the other. Surprisingly, trehalose accumulation correlated with spray-drying survival and glycine betaine accumulation with freeze-drying. This first report showing the ability to modulate the trehalose/GB ratio in osmoprotectants accumulated by a probiotic bacterium opens new perspectives for the optimization of probiotics production.
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spelling pubmed-70629052020-03-23 Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying Gaucher, Floriane Rabah, Houem Kponouglo, Koffigan Bonnassie, Sylvie Pottier, Sandrine Dolivet, Anne Marchand, Pierre Jeantet, Romain Blanc, Philippe Jan, Gwénaël Appl Microbiol Biotechnol Applied Microbial and Cell Physiology Propionibacterium freudenreichii is a beneficial bacterium widely used in food as a probiotic and as a cheese-ripening starter. In these different applications, it is produced, dried, and stored before being used. Both freeze-drying and spray-drying were considered for this purpose. Freeze-drying is a discontinuous process that is energy-consuming but that allows high cell survival. Spray-drying is a continuous process that is more energy-efficient but that can lead to massive bacterial death related to heat, osmotic, and oxidative stresses. We have shown that P. freudenreichii cultivated in hyperconcentrated rich media can be spray-dried with limited bacterial death. However, the general stress tolerance conferred by this hyperosmotic constraint remained a black box. In this study, we modulated P. freudenreichii growth conditions and monitored both osmoprotectant accumulation and stress tolerance acquisition. Changing the ratio between the carbohydrates provided and non-protein nitrogen during growth under osmotic constraint modulated osmoprotectant accumulation. This, in turn, was correlated with P. freudenreichii tolerance towards different stresses, on the one hand, and towards freeze-drying and spray-drying, on the other. Surprisingly, trehalose accumulation correlated with spray-drying survival and glycine betaine accumulation with freeze-drying. This first report showing the ability to modulate the trehalose/GB ratio in osmoprotectants accumulated by a probiotic bacterium opens new perspectives for the optimization of probiotics production. Springer Berlin Heidelberg 2020-02-19 2020 /pmc/articles/PMC7062905/ /pubmed/32076782 http://dx.doi.org/10.1007/s00253-020-10425-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Applied Microbial and Cell Physiology
Gaucher, Floriane
Rabah, Houem
Kponouglo, Koffigan
Bonnassie, Sylvie
Pottier, Sandrine
Dolivet, Anne
Marchand, Pierre
Jeantet, Romain
Blanc, Philippe
Jan, Gwénaël
Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title_full Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title_fullStr Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title_full_unstemmed Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title_short Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying
title_sort intracellular osmoprotectant concentrations determine propionibacterium freudenreichii survival during drying
topic Applied Microbial and Cell Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062905/
https://www.ncbi.nlm.nih.gov/pubmed/32076782
http://dx.doi.org/10.1007/s00253-020-10425-1
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