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Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula

Under nitrogen-limiting conditions, legumes are able to interact symbiotically with bacteria of the Rhizobiaceae family. This interaction gives rise to a new organ, named a root nodule. Root nodules are characterized by an increased glutathione (GSH) and homoglutathione (hGSH) content compared to ro...

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Autores principales: Yang, Li, El Msehli, Sarra, Benyamina, Sofiane, Lambert, Annie, Hopkins, Julie, Cazareth, Julie, Pierre, Olivier, Hérouart, Didier, Achi-Smiti, Samira, Boncompagni, Eric, Frendo, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063052/
https://www.ncbi.nlm.nih.gov/pubmed/32194584
http://dx.doi.org/10.3389/fpls.2020.00137
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author Yang, Li
El Msehli, Sarra
Benyamina, Sofiane
Lambert, Annie
Hopkins, Julie
Cazareth, Julie
Pierre, Olivier
Hérouart, Didier
Achi-Smiti, Samira
Boncompagni, Eric
Frendo, Pierre
author_facet Yang, Li
El Msehli, Sarra
Benyamina, Sofiane
Lambert, Annie
Hopkins, Julie
Cazareth, Julie
Pierre, Olivier
Hérouart, Didier
Achi-Smiti, Samira
Boncompagni, Eric
Frendo, Pierre
author_sort Yang, Li
collection PubMed
description Under nitrogen-limiting conditions, legumes are able to interact symbiotically with bacteria of the Rhizobiaceae family. This interaction gives rise to a new organ, named a root nodule. Root nodules are characterized by an increased glutathione (GSH) and homoglutathione (hGSH) content compared to roots. These low molecular thiols are very important in the biological nitrogen fixation. In order to characterize the modification of nodule activity induced by the microsymbiont glutathione deficiency, physiological, biochemical, and gene expression modifications were analyzed in nodules after the inoculation of Medicago truncatula with the SmgshB mutant of Sinorhizobium meliloti which is deficient in GSH production. The decline in nitrogen fixation efficiency was correlated to the reduction in plant shoot biomass. Flow cytometry analysis showed that SmgshB bacteroids present a higher DNA content than free living bacteria. Live/dead microscopic analysis showed an early bacteroid degradation in SmgshB nodules compared to control nodules which is correlated to a lower bacteroid content at 20 dpi. Finally, the expression of two marker genes involved in nitrogen fixation metabolism, Leghemoglobin and Nodule Cysteine Rich Peptide 001, decreased significantly in mutant nodules at 20 dpi. In contrast, the expression of two marker genes involved in the nodule senescence, Cysteine Protease 6 and Purple Acid Protease, increased significantly in mutant nodules at 10 dpi strengthening the idea that an early senescence process occurs in SmgshB nodules. In conclusion, our results showed that bacterial GSH deficiency does not impair bacterial differentiation but induces an early nodule senescence.
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spelling pubmed-70630522020-03-19 Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula Yang, Li El Msehli, Sarra Benyamina, Sofiane Lambert, Annie Hopkins, Julie Cazareth, Julie Pierre, Olivier Hérouart, Didier Achi-Smiti, Samira Boncompagni, Eric Frendo, Pierre Front Plant Sci Plant Science Under nitrogen-limiting conditions, legumes are able to interact symbiotically with bacteria of the Rhizobiaceae family. This interaction gives rise to a new organ, named a root nodule. Root nodules are characterized by an increased glutathione (GSH) and homoglutathione (hGSH) content compared to roots. These low molecular thiols are very important in the biological nitrogen fixation. In order to characterize the modification of nodule activity induced by the microsymbiont glutathione deficiency, physiological, biochemical, and gene expression modifications were analyzed in nodules after the inoculation of Medicago truncatula with the SmgshB mutant of Sinorhizobium meliloti which is deficient in GSH production. The decline in nitrogen fixation efficiency was correlated to the reduction in plant shoot biomass. Flow cytometry analysis showed that SmgshB bacteroids present a higher DNA content than free living bacteria. Live/dead microscopic analysis showed an early bacteroid degradation in SmgshB nodules compared to control nodules which is correlated to a lower bacteroid content at 20 dpi. Finally, the expression of two marker genes involved in nitrogen fixation metabolism, Leghemoglobin and Nodule Cysteine Rich Peptide 001, decreased significantly in mutant nodules at 20 dpi. In contrast, the expression of two marker genes involved in the nodule senescence, Cysteine Protease 6 and Purple Acid Protease, increased significantly in mutant nodules at 10 dpi strengthening the idea that an early senescence process occurs in SmgshB nodules. In conclusion, our results showed that bacterial GSH deficiency does not impair bacterial differentiation but induces an early nodule senescence. Frontiers Media S.A. 2020-03-03 /pmc/articles/PMC7063052/ /pubmed/32194584 http://dx.doi.org/10.3389/fpls.2020.00137 Text en Copyright © 2020 Yang, El Msehli, Benyamina, Lambert, Hopkins, Cazareth, Pierre, Hérouart, Achi-Smiti, Boncompagni and Frendo http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Li
El Msehli, Sarra
Benyamina, Sofiane
Lambert, Annie
Hopkins, Julie
Cazareth, Julie
Pierre, Olivier
Hérouart, Didier
Achi-Smiti, Samira
Boncompagni, Eric
Frendo, Pierre
Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title_full Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title_fullStr Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title_full_unstemmed Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title_short Glutathione Deficiency in Sinorhizobium meliloti Does Not Impair Bacteroid Differentiation But Induces Early Senescence in the Interaction With Medicago truncatula
title_sort glutathione deficiency in sinorhizobium meliloti does not impair bacteroid differentiation but induces early senescence in the interaction with medicago truncatula
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063052/
https://www.ncbi.nlm.nih.gov/pubmed/32194584
http://dx.doi.org/10.3389/fpls.2020.00137
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