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Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow

The analysis of environmental microplastic particles using FTIR microscopy is a challenging task, due to the very high number of individual particles within a single sample. Therefore, automatable, fast and robust approaches are highly requested. Micro particles were commonly enriched on filters, an...

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Detalles Bibliográficos
Autores principales: Renner, Gerrit, Schmidt, Torsten C., Schram, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063176/
https://www.ncbi.nlm.nih.gov/pubmed/32181150
http://dx.doi.org/10.1016/j.mex.2019.11.015
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author Renner, Gerrit
Schmidt, Torsten C.
Schram, Jürgen
author_facet Renner, Gerrit
Schmidt, Torsten C.
Schram, Jürgen
author_sort Renner, Gerrit
collection PubMed
description The analysis of environmental microplastic particles using FTIR microscopy is a challenging task, due to the very high number of individual particles within a single sample. Therefore, automatable, fast and robust approaches are highly requested. Micro particles were commonly enriched on filters, and sub- or the whole filter area was investigated, which took more than 20h and produced millions of data, which had to be evaluated. This paper presents a new approach of such filter area analysis using an intelligent algorithm to measure only those spots on a filter that would produce evaluable FTIR data. Empty spaces or IR absorbers like carbon black particles were not measured which successfully reduced the total analysis time from 50h to 7h. The presented method is based on system independent Python workflow and can easily be implemented on other FTIR systems. • Fast and intelligent FTIR microscopy area mapping without FPA detector; • Total time reduction from 50 h to 7 h; • Platform independent approach based on Python.
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spelling pubmed-70631762020-03-16 Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow Renner, Gerrit Schmidt, Torsten C. Schram, Jürgen MethodsX Article(s) from the Special Issue on Microplastics analysis The analysis of environmental microplastic particles using FTIR microscopy is a challenging task, due to the very high number of individual particles within a single sample. Therefore, automatable, fast and robust approaches are highly requested. Micro particles were commonly enriched on filters, and sub- or the whole filter area was investigated, which took more than 20h and produced millions of data, which had to be evaluated. This paper presents a new approach of such filter area analysis using an intelligent algorithm to measure only those spots on a filter that would produce evaluable FTIR data. Empty spaces or IR absorbers like carbon black particles were not measured which successfully reduced the total analysis time from 50h to 7h. The presented method is based on system independent Python workflow and can easily be implemented on other FTIR systems. • Fast and intelligent FTIR microscopy area mapping without FPA detector; • Total time reduction from 50 h to 7 h; • Platform independent approach based on Python. Elsevier 2019-12-13 /pmc/articles/PMC7063176/ /pubmed/32181150 http://dx.doi.org/10.1016/j.mex.2019.11.015 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article(s) from the Special Issue on Microplastics analysis
Renner, Gerrit
Schmidt, Torsten C.
Schram, Jürgen
Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title_full Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title_fullStr Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title_full_unstemmed Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title_short Automated rapid & intelligent microplastics mapping by FTIR microscopy: A Python–based workflow
title_sort automated rapid & intelligent microplastics mapping by ftir microscopy: a python–based workflow
topic Article(s) from the Special Issue on Microplastics analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063176/
https://www.ncbi.nlm.nih.gov/pubmed/32181150
http://dx.doi.org/10.1016/j.mex.2019.11.015
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