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Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway

BACKGROUND: Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of th...

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Autores principales: Hou, Ying, Yuan, Peipei, Fu, Yang, Zhang, Qi, Wei, Yaxin, Gao, Liyuan, Liu, Li, Wang, Xiaolan, Zheng, Xiaoke, Feng, Weisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063193/
https://www.ncbi.nlm.nih.gov/pubmed/32184896
http://dx.doi.org/10.1155/2020/6723204
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author Hou, Ying
Yuan, Peipei
Fu, Yang
Zhang, Qi
Wei, Yaxin
Gao, Liyuan
Liu, Li
Wang, Xiaolan
Zheng, Xiaoke
Feng, Weisheng
author_facet Hou, Ying
Yuan, Peipei
Fu, Yang
Zhang, Qi
Wei, Yaxin
Gao, Liyuan
Liu, Li
Wang, Xiaolan
Zheng, Xiaoke
Feng, Weisheng
author_sort Hou, Ying
collection PubMed
description BACKGROUND: Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of the present study is to elucidate the effect and mechanism of DZBTS on spermatogenic function of a heat stress model in rats. METHODS: Male Wistar rats (280–320 g) were given different doses of DZBTS (0.4853 g/kg/d or 0.9707 g/kg/d), Shengjing capsule (0.56 g/kg/d), or double distilled water for 15 days. A 43°C hot water bath for 30 minutes was used to stimulate the testis of rats. Sperm count, sperm motility, the organ index of kidney and gonadal organs, serum sex hormone levels, and serum oxidising reaction index were measured. Haematoxylin and eosin (HE) staining was used to observe the morphology of the testis and kidney. The expression of Hsp70 in testes was observed by immunofluorescence. The changes in heat stress, reproductive-related protein, and mRNA were measured by western blot assay and RT-qPCR. RESULTS: Heat stress downregulated the levels of sex hormone (P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or CONCLUSIONS: Our results for the first time have found that DZBTS can improve spermatogenesis disorder in a heat stress model in rats, which may be mainly by regulating AR, sperm regulatory protein CREB1, and the HSF/Hsp70 signaling pathway to decrease oxidative stress.
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spelling pubmed-70631932020-03-17 Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway Hou, Ying Yuan, Peipei Fu, Yang Zhang, Qi Wei, Yaxin Gao, Liyuan Liu, Li Wang, Xiaolan Zheng, Xiaoke Feng, Weisheng Evid Based Complement Alternat Med Research Article BACKGROUND: Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of the present study is to elucidate the effect and mechanism of DZBTS on spermatogenic function of a heat stress model in rats. METHODS: Male Wistar rats (280–320 g) were given different doses of DZBTS (0.4853 g/kg/d or 0.9707 g/kg/d), Shengjing capsule (0.56 g/kg/d), or double distilled water for 15 days. A 43°C hot water bath for 30 minutes was used to stimulate the testis of rats. Sperm count, sperm motility, the organ index of kidney and gonadal organs, serum sex hormone levels, and serum oxidising reaction index were measured. Haematoxylin and eosin (HE) staining was used to observe the morphology of the testis and kidney. The expression of Hsp70 in testes was observed by immunofluorescence. The changes in heat stress, reproductive-related protein, and mRNA were measured by western blot assay and RT-qPCR. RESULTS: Heat stress downregulated the levels of sex hormone (P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or P < 0.05 or CONCLUSIONS: Our results for the first time have found that DZBTS can improve spermatogenesis disorder in a heat stress model in rats, which may be mainly by regulating AR, sperm regulatory protein CREB1, and the HSF/Hsp70 signaling pathway to decrease oxidative stress. Hindawi 2020-02-27 /pmc/articles/PMC7063193/ /pubmed/32184896 http://dx.doi.org/10.1155/2020/6723204 Text en Copyright © 2020 Ying Hou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hou, Ying
Yuan, Peipei
Fu, Yang
Zhang, Qi
Wei, Yaxin
Gao, Liyuan
Liu, Li
Wang, Xiaolan
Zheng, Xiaoke
Feng, Weisheng
Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title_full Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title_fullStr Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title_full_unstemmed Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title_short Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
title_sort duzhong butiansu prescription improves heat stress-induced spermatogenic dysfunction by regulating sperm formation and heat stress pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063193/
https://www.ncbi.nlm.nih.gov/pubmed/32184896
http://dx.doi.org/10.1155/2020/6723204
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