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The HARE chip for efficient time-resolved serial synchrotron crystallography

Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the ‘hit-and-return’ (HARE) protocol allows for efficient time-resolved data collection. The specific patter...

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Detalles Bibliográficos
Autores principales: Mehrabi, Pedram, Müller-Werkmeister, Henrike M., Leimkohl, Jan-Philipp, Schikora, Hendrik, Ninkovic, Jelena, Krivokuca, Silvia, Andriček, Ladislav, Epp, Sascha W., Sherrell, Darren, Owen, Robin L., Pearson, Arwen R., Tellkamp, Friedjof, Schulz, Eike C., Miller, R. J. Dwayne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064102/
https://www.ncbi.nlm.nih.gov/pubmed/32153274
http://dx.doi.org/10.1107/S1600577520000685
Descripción
Sumario:Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the ‘hit-and-return’ (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.