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Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass

The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydr...

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Detalles Bibliográficos
Autores principales: Lapeña, David, Olsen, Pernille M., Arntzen, Magnus Ø., Kosa, Gergely, Passoth, Volkmar, Eijsink, Vincent G. H., Horn, Svein J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064453/
https://www.ncbi.nlm.nih.gov/pubmed/31883034
http://dx.doi.org/10.1007/s00449-019-02271-x
Descripción
Sumario:The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m(3)) continuously operated fermenters to replace 10% of the fish feed protein used in Norway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00449-019-02271-x) contains supplementary material, which is available to authorized users.