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Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes

In the CRISPR-Cas systems, Cas13a is an RNA-guided RNA nuclease specifically targeting single strand RNA. We developed a Cas13a mediated CRISPR interference tool to target mRNA for gene silencing in mosquitoes. A Cas13a expressing plasmid was delivered to mosquitoes by intrathoracic injection, and C...

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Detalles Bibliográficos
Autores principales:	Kulkarni, Aditi, Yu, Wanqin, Moon, Alex S., Pandey, Ashmita, Hanley, Kathryn A., Xu, Jiannong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Ivyspring International Publisher 2020
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064736/ https://www.ncbi.nlm.nih.gov/pubmed/32190127 http://dx.doi.org/10.7150/jgen.43928

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