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Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia
Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Copernicus GmbH
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065384/ https://www.ncbi.nlm.nih.gov/pubmed/32175457 http://dx.doi.org/10.5194/aab-61-481-2018 |
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author | Amiri, Sihem Jemmali, Bayrem Ferchichi, Mohamed Amine Jeljeli, Hajer Boulbaba, Rekik Ben Gara, Abderrahmane |
author_facet | Amiri, Sihem Jemmali, Bayrem Ferchichi, Mohamed Amine Jeljeli, Hajer Boulbaba, Rekik Ben Gara, Abderrahmane |
author_sort | Amiri, Sihem |
collection | PubMed |
description | Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI([Formula: see text] // [Formula: see text]) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and ([Formula: see text] / [Formula: see text])//([Formula: see text] / [Formula: see text])//([Formula: see text] / [Formula: see text]) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI([Formula: see text] / [Formula: see text]) cows tended to have a longer CI and DI than MspI([Formula: see text] / [Formula: see text]) and MspI([Formula: see text] / [Formula: see text]) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program. |
format | Online Article Text |
id | pubmed-7065384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Copernicus GmbH |
record_format | MEDLINE/PubMed |
spelling | pubmed-70653842020-03-13 Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia Amiri, Sihem Jemmali, Bayrem Ferchichi, Mohamed Amine Jeljeli, Hajer Boulbaba, Rekik Ben Gara, Abderrahmane Arch Anim Breed Original Study Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI([Formula: see text] // [Formula: see text]) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and ([Formula: see text] / [Formula: see text])//([Formula: see text] / [Formula: see text])//([Formula: see text] / [Formula: see text]) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI([Formula: see text] / [Formula: see text]) cows tended to have a longer CI and DI than MspI([Formula: see text] / [Formula: see text]) and MspI([Formula: see text] / [Formula: see text]) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program. Copernicus GmbH 2018-12-19 /pmc/articles/PMC7065384/ /pubmed/32175457 http://dx.doi.org/10.5194/aab-61-481-2018 Text en Copyright: © 2018 Sihem Amiri et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Study Amiri, Sihem Jemmali, Bayrem Ferchichi, Mohamed Amine Jeljeli, Hajer Boulbaba, Rekik Ben Gara, Abderrahmane Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title | Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title_full | Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title_fullStr | Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title_full_unstemmed | Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title_short | Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia |
title_sort | assessment of growth hormone gene polymorphism effects on reproductive traits in holstein dairy cattle in tunisia |
topic | Original Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065384/ https://www.ncbi.nlm.nih.gov/pubmed/32175457 http://dx.doi.org/10.5194/aab-61-481-2018 |
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