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Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region
In this research, Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region was surveyed by DNA sequencing in some of the native sheep breeds that are reared in Turkey. A total of 80 samples were collected from eight different Turkish native sheep breeds, and these samples were used f...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Copernicus GmbH
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065386/ https://www.ncbi.nlm.nih.gov/pubmed/32175456 http://dx.doi.org/10.5194/aab-61-475-2018 |
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author | Özdil, Fulya İlhan, Fatma Işık, Raziye |
author_facet | Özdil, Fulya İlhan, Fatma Işık, Raziye |
author_sort | Özdil, Fulya |
collection | PubMed |
description | In this research, Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region was surveyed by DNA sequencing in some of the native sheep breeds that are reared in Turkey. A total of 80 samples were collected from eight different Turkish native sheep breeds, and these samples were used for DNA sequencing. The exon 2 region of Ovar-DRB1 in the MHC gene region was polymerase chain reaction (PCR) amplified and sequenced. A total of 25 new alleles were revealed in the Ovar-DRB1 gene in Turkish native sheep breeds with 24 variable sites; only 13 sites were parsimony informative. The average pairwise genetic distance was 0.029 % for the Ovar-DRB1 gene exon 2 region. The sequence variations at eight different positions (7026, 7036, 7040, 7053, 7059, 7069, 7131 and 7214) are found in all of the studied samples. G [Formula: see text] C transversion at position 7081 is only seen in Akkaraman sheep breed, whereas T [Formula: see text] C transition at position 7097 is only seen in one sample from the Akkaraman breed. Overall, two main groups were detected among the 25 alleles from Turkish native sheep breeds. All Daǧliç and Kivircik alleles and one allele from Karayaka, Malya and Sakiz are grouped together while all the other breeds are grouped in the other branch. |
format | Online Article Text |
id | pubmed-7065386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Copernicus GmbH |
record_format | MEDLINE/PubMed |
spelling | pubmed-70653862020-03-13 Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region Özdil, Fulya İlhan, Fatma Işık, Raziye Arch Anim Breed Original Study In this research, Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region was surveyed by DNA sequencing in some of the native sheep breeds that are reared in Turkey. A total of 80 samples were collected from eight different Turkish native sheep breeds, and these samples were used for DNA sequencing. The exon 2 region of Ovar-DRB1 in the MHC gene region was polymerase chain reaction (PCR) amplified and sequenced. A total of 25 new alleles were revealed in the Ovar-DRB1 gene in Turkish native sheep breeds with 24 variable sites; only 13 sites were parsimony informative. The average pairwise genetic distance was 0.029 % for the Ovar-DRB1 gene exon 2 region. The sequence variations at eight different positions (7026, 7036, 7040, 7053, 7059, 7069, 7131 and 7214) are found in all of the studied samples. G [Formula: see text] C transversion at position 7081 is only seen in Akkaraman sheep breed, whereas T [Formula: see text] C transition at position 7097 is only seen in one sample from the Akkaraman breed. Overall, two main groups were detected among the 25 alleles from Turkish native sheep breeds. All Daǧliç and Kivircik alleles and one allele from Karayaka, Malya and Sakiz are grouped together while all the other breeds are grouped in the other branch. Copernicus GmbH 2018-12-06 /pmc/articles/PMC7065386/ /pubmed/32175456 http://dx.doi.org/10.5194/aab-61-475-2018 Text en Copyright: © 2018 Fulya Özdil et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Study Özdil, Fulya İlhan, Fatma Işık, Raziye Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title | Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title_full | Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title_fullStr | Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title_full_unstemmed | Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title_short | Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region |
title_sort | genetic characterization of some turkish sheep breeds based on the sequencing of the ovar-drb1 gene in the major histocompatibility complex (mhc) gene region |
topic | Original Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065386/ https://www.ncbi.nlm.nih.gov/pubmed/32175456 http://dx.doi.org/10.5194/aab-61-475-2018 |
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