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Multispectral near infrared absorption imaging for histology of skin cancer

Multispectral imaging combines the spectral resolution of spectroscopy with the spatial resolution of imaging and is therefore very useful for biomedical applications. Currently, histological diagnostics use mainly stainings with standard dyes (eg, hematoxylin + eosin) to identify tumors. This metho...

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Autores principales: Spreinat, Alexander, Selvaggio, Gabriele, Erpenbeck, Luise, Kruss, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY‐VCH Verlag GmbH & Co. KGaA 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065629/
https://www.ncbi.nlm.nih.gov/pubmed/31602799
http://dx.doi.org/10.1002/jbio.201960080
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author Spreinat, Alexander
Selvaggio, Gabriele
Erpenbeck, Luise
Kruss, Sebastian
author_facet Spreinat, Alexander
Selvaggio, Gabriele
Erpenbeck, Luise
Kruss, Sebastian
author_sort Spreinat, Alexander
collection PubMed
description Multispectral imaging combines the spectral resolution of spectroscopy with the spatial resolution of imaging and is therefore very useful for biomedical applications. Currently, histological diagnostics use mainly stainings with standard dyes (eg, hematoxylin + eosin) to identify tumors. This method is not applicable in vivo and provides low amounts of chemical information. Biomolecules absorb near infrared light (NIR, 800‐1700 nm) at different wavelengths, which could be used to fingerprint tissue. Here, we built a NIR multispectral absorption imaging setup to study skin tissue samples. NIR light (900‐1500 nm) was used for homogenous wide‐field transmission illumination and detected by a cooled InGaAs camera. In this setup, images I(x, y, λ) from dermatological samples (melanoma, nodular basal‐cell carcinoma, squamous‐cell carcinoma) were acquired to distinguish healthy from diseased tissue regions. In summary, we show the potential of multispectral NIR imaging for cancer diagnostics. [Image: see text]
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spelling pubmed-70656292020-03-16 Multispectral near infrared absorption imaging for histology of skin cancer Spreinat, Alexander Selvaggio, Gabriele Erpenbeck, Luise Kruss, Sebastian J Biophotonics Full Articles Multispectral imaging combines the spectral resolution of spectroscopy with the spatial resolution of imaging and is therefore very useful for biomedical applications. Currently, histological diagnostics use mainly stainings with standard dyes (eg, hematoxylin + eosin) to identify tumors. This method is not applicable in vivo and provides low amounts of chemical information. Biomolecules absorb near infrared light (NIR, 800‐1700 nm) at different wavelengths, which could be used to fingerprint tissue. Here, we built a NIR multispectral absorption imaging setup to study skin tissue samples. NIR light (900‐1500 nm) was used for homogenous wide‐field transmission illumination and detected by a cooled InGaAs camera. In this setup, images I(x, y, λ) from dermatological samples (melanoma, nodular basal‐cell carcinoma, squamous‐cell carcinoma) were acquired to distinguish healthy from diseased tissue regions. In summary, we show the potential of multispectral NIR imaging for cancer diagnostics. [Image: see text] WILEY‐VCH Verlag GmbH & Co. KGaA 2019-11-05 2020-01 /pmc/articles/PMC7065629/ /pubmed/31602799 http://dx.doi.org/10.1002/jbio.201960080 Text en © 2019 The Authors. Journal of Biophotonics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Articles
Spreinat, Alexander
Selvaggio, Gabriele
Erpenbeck, Luise
Kruss, Sebastian
Multispectral near infrared absorption imaging for histology of skin cancer
title Multispectral near infrared absorption imaging for histology of skin cancer
title_full Multispectral near infrared absorption imaging for histology of skin cancer
title_fullStr Multispectral near infrared absorption imaging for histology of skin cancer
title_full_unstemmed Multispectral near infrared absorption imaging for histology of skin cancer
title_short Multispectral near infrared absorption imaging for histology of skin cancer
title_sort multispectral near infrared absorption imaging for histology of skin cancer
topic Full Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065629/
https://www.ncbi.nlm.nih.gov/pubmed/31602799
http://dx.doi.org/10.1002/jbio.201960080
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