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Lightsheet fluorescence lifetime imaging microscopy with wide‐field time‐correlated single photon counting
We report on wide‐field time‐correlated single photon counting (TCSPC)‐based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single‐photon sensitive camera, is u...
Autores principales: | Hirvonen, Liisa M., Nedbal, Jakub, Almutairi, Norah, Phillips, Thomas A., Becker, Wolfgang, Conneely, Thomas, Milnes, James, Cox, Susan, Stürzenbaum, Stephen, Suhling, Klaus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY‐VCH Verlag GmbH & Co. KGaA
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065631/ https://www.ncbi.nlm.nih.gov/pubmed/31661595 http://dx.doi.org/10.1002/jbio.201960099 |
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