Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples

We established an ultrasensitive method for identifying multiple enzymes in biological samples by using a multiplexed microdevice-based single-molecule enzymatic assay. We used a paradigm in which we “count” the number of enzyme molecules by profiling their single enzyme activity characteristics tow...

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Detalles Bibliográficos
Autores principales: Sakamoto, Shingo, Komatsu, Toru, Watanabe, Rikiya, Zhang, Yi, Inoue, Taiki, Kawaguchi, Mitsuyasu, Nakagawa, Hidehiko, Ueno, Takaaki, Okusaka, Takuji, Honda, Kazufumi, Noji, Hiroyuki, Urano, Yasuteru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065886/
https://www.ncbi.nlm.nih.gov/pubmed/32195342
http://dx.doi.org/10.1126/sciadv.aay0888

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