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Antigenic characterization of novel H1 influenza A viruses in swine
Novel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and ant...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066140/ https://www.ncbi.nlm.nih.gov/pubmed/32161289 http://dx.doi.org/10.1038/s41598-020-61315-5 |
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author | Tapia, Rodrigo Torremorell, Montserrat Culhane, Marie Medina, Rafael A. Neira, Víctor |
author_facet | Tapia, Rodrigo Torremorell, Montserrat Culhane, Marie Medina, Rafael A. Neira, Víctor |
author_sort | Tapia, Rodrigo |
collection | PubMed |
description | Novel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and antigenic analysis by hemagglutination inhibition assay were carried out. Three antigenic clusters were identified, named Chilean H1 A (ChH1A), Chilean H1 B (ChH1B), and A(H1N1)pdm09-like. The antigenic sites of ChH1A and ChH1B strains were 10–60% distant from those of commercial vaccine strains at the amino acid sequence level. Antigenic variants were identified within the clusters ChH1A and A(H1N1)pdm09-like. Substitutions in the main antigenic sites (E153G in Sa, Q193H in Sb, D168N in Ca1, P137S in Ca2, and F71L in Cb) were detected in variants from the ChH1A cluster, whereas only a single substitution in antigenic site Sa (G155E) was detected in variants from A(H1N1)pdm09-like cluster, which confirms the importance to carrying out antigenic analyses in addition to genetic analyses to evaluate control measures such as vaccination. These results highlight the need to update vaccines for swine in Chile and the importance of continued surveillance to determine the onward transmission of antigenic variants in Chilean pig populations. |
format | Online Article Text |
id | pubmed-7066140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70661402020-03-19 Antigenic characterization of novel H1 influenza A viruses in swine Tapia, Rodrigo Torremorell, Montserrat Culhane, Marie Medina, Rafael A. Neira, Víctor Sci Rep Article Novel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and antigenic analysis by hemagglutination inhibition assay were carried out. Three antigenic clusters were identified, named Chilean H1 A (ChH1A), Chilean H1 B (ChH1B), and A(H1N1)pdm09-like. The antigenic sites of ChH1A and ChH1B strains were 10–60% distant from those of commercial vaccine strains at the amino acid sequence level. Antigenic variants were identified within the clusters ChH1A and A(H1N1)pdm09-like. Substitutions in the main antigenic sites (E153G in Sa, Q193H in Sb, D168N in Ca1, P137S in Ca2, and F71L in Cb) were detected in variants from the ChH1A cluster, whereas only a single substitution in antigenic site Sa (G155E) was detected in variants from A(H1N1)pdm09-like cluster, which confirms the importance to carrying out antigenic analyses in addition to genetic analyses to evaluate control measures such as vaccination. These results highlight the need to update vaccines for swine in Chile and the importance of continued surveillance to determine the onward transmission of antigenic variants in Chilean pig populations. Nature Publishing Group UK 2020-03-11 /pmc/articles/PMC7066140/ /pubmed/32161289 http://dx.doi.org/10.1038/s41598-020-61315-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tapia, Rodrigo Torremorell, Montserrat Culhane, Marie Medina, Rafael A. Neira, Víctor Antigenic characterization of novel H1 influenza A viruses in swine |
title | Antigenic characterization of novel H1 influenza A viruses in swine |
title_full | Antigenic characterization of novel H1 influenza A viruses in swine |
title_fullStr | Antigenic characterization of novel H1 influenza A viruses in swine |
title_full_unstemmed | Antigenic characterization of novel H1 influenza A viruses in swine |
title_short | Antigenic characterization of novel H1 influenza A viruses in swine |
title_sort | antigenic characterization of novel h1 influenza a viruses in swine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066140/ https://www.ncbi.nlm.nih.gov/pubmed/32161289 http://dx.doi.org/10.1038/s41598-020-61315-5 |
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