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An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric ca...

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Autores principales: Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L., Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H. D., Visser, Lindy L., Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A. M., Kemmeren, Patrick, de Krijger, Ronald R., Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C., van den Heuvel-Eibrink, Marry M., Molenaar, Jan J., van Boxtel, Ruben, Holstege, Frank C. P., Clevers, Hans, Drost, Jarno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066173/
https://www.ncbi.nlm.nih.gov/pubmed/32161258
http://dx.doi.org/10.1038/s41467-020-15155-6
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author Calandrini, Camilla
Schutgens, Frans
Oka, Rurika
Margaritis, Thanasis
Candelli, Tito
Mathijsen, Luka
Ammerlaan, Carola
van Ineveld, Ravian L.
Derakhshan, Sepide
de Haan, Sanne
Dolman, Emmy
Lijnzaad, Philip
Custers, Lars
Begthel, Harry
Kerstens, Hindrik H. D.
Visser, Lindy L.
Rookmaaker, Maarten
Verhaar, Marianne
Tytgat, Godelieve A. M.
Kemmeren, Patrick
de Krijger, Ronald R.
Al-Saadi, Reem
Pritchard-Jones, Kathy
Kool, Marcel
Rios, Anne C.
van den Heuvel-Eibrink, Marry M.
Molenaar, Jan J.
van Boxtel, Ruben
Holstege, Frank C. P.
Clevers, Hans
Drost, Jarno
author_facet Calandrini, Camilla
Schutgens, Frans
Oka, Rurika
Margaritis, Thanasis
Candelli, Tito
Mathijsen, Luka
Ammerlaan, Carola
van Ineveld, Ravian L.
Derakhshan, Sepide
de Haan, Sanne
Dolman, Emmy
Lijnzaad, Philip
Custers, Lars
Begthel, Harry
Kerstens, Hindrik H. D.
Visser, Lindy L.
Rookmaaker, Maarten
Verhaar, Marianne
Tytgat, Godelieve A. M.
Kemmeren, Patrick
de Krijger, Ronald R.
Al-Saadi, Reem
Pritchard-Jones, Kathy
Kool, Marcel
Rios, Anne C.
van den Heuvel-Eibrink, Marry M.
Molenaar, Jan J.
van Boxtel, Ruben
Holstege, Frank C. P.
Clevers, Hans
Drost, Jarno
author_sort Calandrini, Camilla
collection PubMed
description Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.
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spelling pubmed-70661732020-03-18 An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity Calandrini, Camilla Schutgens, Frans Oka, Rurika Margaritis, Thanasis Candelli, Tito Mathijsen, Luka Ammerlaan, Carola van Ineveld, Ravian L. Derakhshan, Sepide de Haan, Sanne Dolman, Emmy Lijnzaad, Philip Custers, Lars Begthel, Harry Kerstens, Hindrik H. D. Visser, Lindy L. Rookmaaker, Maarten Verhaar, Marianne Tytgat, Godelieve A. M. Kemmeren, Patrick de Krijger, Ronald R. Al-Saadi, Reem Pritchard-Jones, Kathy Kool, Marcel Rios, Anne C. van den Heuvel-Eibrink, Marry M. Molenaar, Jan J. van Boxtel, Ruben Holstege, Frank C. P. Clevers, Hans Drost, Jarno Nat Commun Article Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine. Nature Publishing Group UK 2020-03-11 /pmc/articles/PMC7066173/ /pubmed/32161258 http://dx.doi.org/10.1038/s41467-020-15155-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Calandrini, Camilla
Schutgens, Frans
Oka, Rurika
Margaritis, Thanasis
Candelli, Tito
Mathijsen, Luka
Ammerlaan, Carola
van Ineveld, Ravian L.
Derakhshan, Sepide
de Haan, Sanne
Dolman, Emmy
Lijnzaad, Philip
Custers, Lars
Begthel, Harry
Kerstens, Hindrik H. D.
Visser, Lindy L.
Rookmaaker, Maarten
Verhaar, Marianne
Tytgat, Godelieve A. M.
Kemmeren, Patrick
de Krijger, Ronald R.
Al-Saadi, Reem
Pritchard-Jones, Kathy
Kool, Marcel
Rios, Anne C.
van den Heuvel-Eibrink, Marry M.
Molenaar, Jan J.
van Boxtel, Ruben
Holstege, Frank C. P.
Clevers, Hans
Drost, Jarno
An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title_full An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title_fullStr An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title_full_unstemmed An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title_short An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
title_sort organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066173/
https://www.ncbi.nlm.nih.gov/pubmed/32161258
http://dx.doi.org/10.1038/s41467-020-15155-6
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