Cargando…
An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric ca...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066173/ https://www.ncbi.nlm.nih.gov/pubmed/32161258 http://dx.doi.org/10.1038/s41467-020-15155-6 |
_version_ | 1783505191702626304 |
---|---|
author | Calandrini, Camilla Schutgens, Frans Oka, Rurika Margaritis, Thanasis Candelli, Tito Mathijsen, Luka Ammerlaan, Carola van Ineveld, Ravian L. Derakhshan, Sepide de Haan, Sanne Dolman, Emmy Lijnzaad, Philip Custers, Lars Begthel, Harry Kerstens, Hindrik H. D. Visser, Lindy L. Rookmaaker, Maarten Verhaar, Marianne Tytgat, Godelieve A. M. Kemmeren, Patrick de Krijger, Ronald R. Al-Saadi, Reem Pritchard-Jones, Kathy Kool, Marcel Rios, Anne C. van den Heuvel-Eibrink, Marry M. Molenaar, Jan J. van Boxtel, Ruben Holstege, Frank C. P. Clevers, Hans Drost, Jarno |
author_facet | Calandrini, Camilla Schutgens, Frans Oka, Rurika Margaritis, Thanasis Candelli, Tito Mathijsen, Luka Ammerlaan, Carola van Ineveld, Ravian L. Derakhshan, Sepide de Haan, Sanne Dolman, Emmy Lijnzaad, Philip Custers, Lars Begthel, Harry Kerstens, Hindrik H. D. Visser, Lindy L. Rookmaaker, Maarten Verhaar, Marianne Tytgat, Godelieve A. M. Kemmeren, Patrick de Krijger, Ronald R. Al-Saadi, Reem Pritchard-Jones, Kathy Kool, Marcel Rios, Anne C. van den Heuvel-Eibrink, Marry M. Molenaar, Jan J. van Boxtel, Ruben Holstege, Frank C. P. Clevers, Hans Drost, Jarno |
author_sort | Calandrini, Camilla |
collection | PubMed |
description | Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine. |
format | Online Article Text |
id | pubmed-7066173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70661732020-03-18 An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity Calandrini, Camilla Schutgens, Frans Oka, Rurika Margaritis, Thanasis Candelli, Tito Mathijsen, Luka Ammerlaan, Carola van Ineveld, Ravian L. Derakhshan, Sepide de Haan, Sanne Dolman, Emmy Lijnzaad, Philip Custers, Lars Begthel, Harry Kerstens, Hindrik H. D. Visser, Lindy L. Rookmaaker, Maarten Verhaar, Marianne Tytgat, Godelieve A. M. Kemmeren, Patrick de Krijger, Ronald R. Al-Saadi, Reem Pritchard-Jones, Kathy Kool, Marcel Rios, Anne C. van den Heuvel-Eibrink, Marry M. Molenaar, Jan J. van Boxtel, Ruben Holstege, Frank C. P. Clevers, Hans Drost, Jarno Nat Commun Article Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine. Nature Publishing Group UK 2020-03-11 /pmc/articles/PMC7066173/ /pubmed/32161258 http://dx.doi.org/10.1038/s41467-020-15155-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Calandrini, Camilla Schutgens, Frans Oka, Rurika Margaritis, Thanasis Candelli, Tito Mathijsen, Luka Ammerlaan, Carola van Ineveld, Ravian L. Derakhshan, Sepide de Haan, Sanne Dolman, Emmy Lijnzaad, Philip Custers, Lars Begthel, Harry Kerstens, Hindrik H. D. Visser, Lindy L. Rookmaaker, Maarten Verhaar, Marianne Tytgat, Godelieve A. M. Kemmeren, Patrick de Krijger, Ronald R. Al-Saadi, Reem Pritchard-Jones, Kathy Kool, Marcel Rios, Anne C. van den Heuvel-Eibrink, Marry M. Molenaar, Jan J. van Boxtel, Ruben Holstege, Frank C. P. Clevers, Hans Drost, Jarno An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title | An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title_full | An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title_fullStr | An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title_full_unstemmed | An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title_short | An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
title_sort | organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066173/ https://www.ncbi.nlm.nih.gov/pubmed/32161258 http://dx.doi.org/10.1038/s41467-020-15155-6 |
work_keys_str_mv | AT calandrinicamilla anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT schutgensfrans anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT okarurika anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT margaritisthanasis anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT candellitito anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT mathijsenluka anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT ammerlaancarola anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vanineveldravianl anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT derakhshansepide anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dehaansanne anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dolmanemmy anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT lijnzaadphilip anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT custerslars anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT begthelharry anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT kerstenshindrikhd anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT visserlindyl anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT rookmaakermaarten anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT verhaarmarianne anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT tytgatgodelieveam anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT kemmerenpatrick anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dekrijgerronaldr anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT alsaadireem anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT pritchardjoneskathy anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT koolmarcel anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT riosannec anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vandenheuveleibrinkmarrym anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT molenaarjanj anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vanboxtelruben anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT holstegefrankcp anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT clevershans anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT drostjarno anorganoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT calandrinicamilla organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT schutgensfrans organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT okarurika organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT margaritisthanasis organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT candellitito organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT mathijsenluka organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT ammerlaancarola organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vanineveldravianl organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT derakhshansepide organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dehaansanne organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dolmanemmy organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT lijnzaadphilip organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT custerslars organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT begthelharry organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT kerstenshindrikhd organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT visserlindyl organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT rookmaakermaarten organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT verhaarmarianne organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT tytgatgodelieveam organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT kemmerenpatrick organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT dekrijgerronaldr organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT alsaadireem organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT pritchardjoneskathy organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT koolmarcel organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT riosannec organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vandenheuveleibrinkmarrym organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT molenaarjanj organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT vanboxtelruben organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT holstegefrankcp organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT clevershans organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity AT drostjarno organoidbiobankforchildhoodkidneycancersthatcapturesdiseaseandtissueheterogeneity |