N-Terminal Amino Acids Determine KLF4 Protein Stability in 2A Peptide-Linked Polycistronic Reprogramming Constructs

A common strategy for multi-protein expression is to link genes by self-cleaving 2A peptide sequences. Yet, little is known how the 2A peptide-derived N-terminal proline or adjacent non-native residues introduced during cDNA cloning affects protein stoichiometry. Polycistronic reprogramming construc...

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Detalles Bibliográficos
Autores principales: Reinhardt, Anika, Kagawa, Harunobu, Woltjen, Knut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066363/
https://www.ncbi.nlm.nih.gov/pubmed/32109368
http://dx.doi.org/10.1016/j.stemcr.2020.01.014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066363/
https://www.ncbi.nlm.nih.gov/pubmed/32109368
http://dx.doi.org/10.1016/j.stemcr.2020.01.014

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