Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard

Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here, we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental sample using a synthetic standard in minuscule amou...

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Detalles Bibliográficos
Autores principales: Zemb, Olivier, Achard, Caroline S., Hamelin, Jerome, De Almeida, Marie‐Léa, Gabinaud, Béatrice, Cauquil, Laurent, Verschuren, Lisanne M.G., Godon, Jean‐Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066463/
https://www.ncbi.nlm.nih.gov/pubmed/31927795
http://dx.doi.org/10.1002/mbo3.977

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