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Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy

Toxoplasmosis is a zoonotic food-borne disease caused by Toxoplasma gondii, a land-derived protozoan parasite that infects a broad range of terrestrial and aquatic hosts. T. gondii may reach coastal waters via contaminated freshwater runoff and its oocysts may enter into the marine food web. Marine...

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Autores principales: Santoro, Mario, Viscardi, Maurizio, Boccia, Federica, Borriello, Giorgia, Lucibelli, Maria Gabriella, Auriemma, Clementina, Anastasio, Aniello, Veneziano, Vincenzo, Galiero, Giorgio, Baldi, Loredana, Fusco, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066981/
https://www.ncbi.nlm.nih.gov/pubmed/32210944
http://dx.doi.org/10.3389/fmicb.2020.00355
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author Santoro, Mario
Viscardi, Maurizio
Boccia, Federica
Borriello, Giorgia
Lucibelli, Maria Gabriella
Auriemma, Clementina
Anastasio, Aniello
Veneziano, Vincenzo
Galiero, Giorgio
Baldi, Loredana
Fusco, Giovanna
author_facet Santoro, Mario
Viscardi, Maurizio
Boccia, Federica
Borriello, Giorgia
Lucibelli, Maria Gabriella
Auriemma, Clementina
Anastasio, Aniello
Veneziano, Vincenzo
Galiero, Giorgio
Baldi, Loredana
Fusco, Giovanna
author_sort Santoro, Mario
collection PubMed
description Toxoplasmosis is a zoonotic food-borne disease caused by Toxoplasma gondii, a land-derived protozoan parasite that infects a broad range of terrestrial and aquatic hosts. T. gondii may reach coastal waters via contaminated freshwater runoff and its oocysts may enter into the marine food web. Marine invertebrates as mussels being filter feeders are exposed and may concentrate T. gondii oocysts representing a potential source of infection for animals and humans. The present works investigated the prevalence, parasite burden and genotypes of T. gondii in the Mediterranean mussels (Mytilus galloprovincialis) from southern Italy. We sampled a total of 382 individual Mediterranean mussels from May to August 2018 from seven production sites in the Gulf of Naples (Campania region). An additional sample including 27 farmed Mediterranean mussels was obtained in February 2018 from a mollusk depuration plant in Corigliano Calabro (Calabria region). T. gondii DNA was detected in 43 out of 409 (10.5%) Mediterranean mussels from seven out of eight sampling sites. The number of T. gondii copies/g in the digestive gland ranged from 0.14 to 1.18. Fragment analysis of Short Tandem Repeats (STRs) at 5 microsatellite loci was performed from 10 T. gondii PCR positive samples revealing the presence of five distinct genotypes including one corresponding to type I and four atypical genotypes. These findings suggest potential implications of epidemiological importance for human and animal health because both type I and atypical genotypes could be highly pathogenic.
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spelling pubmed-70669812020-03-24 Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy Santoro, Mario Viscardi, Maurizio Boccia, Federica Borriello, Giorgia Lucibelli, Maria Gabriella Auriemma, Clementina Anastasio, Aniello Veneziano, Vincenzo Galiero, Giorgio Baldi, Loredana Fusco, Giovanna Front Microbiol Microbiology Toxoplasmosis is a zoonotic food-borne disease caused by Toxoplasma gondii, a land-derived protozoan parasite that infects a broad range of terrestrial and aquatic hosts. T. gondii may reach coastal waters via contaminated freshwater runoff and its oocysts may enter into the marine food web. Marine invertebrates as mussels being filter feeders are exposed and may concentrate T. gondii oocysts representing a potential source of infection for animals and humans. The present works investigated the prevalence, parasite burden and genotypes of T. gondii in the Mediterranean mussels (Mytilus galloprovincialis) from southern Italy. We sampled a total of 382 individual Mediterranean mussels from May to August 2018 from seven production sites in the Gulf of Naples (Campania region). An additional sample including 27 farmed Mediterranean mussels was obtained in February 2018 from a mollusk depuration plant in Corigliano Calabro (Calabria region). T. gondii DNA was detected in 43 out of 409 (10.5%) Mediterranean mussels from seven out of eight sampling sites. The number of T. gondii copies/g in the digestive gland ranged from 0.14 to 1.18. Fragment analysis of Short Tandem Repeats (STRs) at 5 microsatellite loci was performed from 10 T. gondii PCR positive samples revealing the presence of five distinct genotypes including one corresponding to type I and four atypical genotypes. These findings suggest potential implications of epidemiological importance for human and animal health because both type I and atypical genotypes could be highly pathogenic. Frontiers Media S.A. 2020-03-05 /pmc/articles/PMC7066981/ /pubmed/32210944 http://dx.doi.org/10.3389/fmicb.2020.00355 Text en Copyright © 2020 Santoro, Viscardi, Boccia, Borriello, Lucibelli, Auriemma, Anastasio, Veneziano, Galiero, Baldi and Fusco. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Santoro, Mario
Viscardi, Maurizio
Boccia, Federica
Borriello, Giorgia
Lucibelli, Maria Gabriella
Auriemma, Clementina
Anastasio, Aniello
Veneziano, Vincenzo
Galiero, Giorgio
Baldi, Loredana
Fusco, Giovanna
Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title_full Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title_fullStr Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title_full_unstemmed Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title_short Parasite Load and STRs Genotyping of Toxoplasma gondii Isolates From Mediterranean Mussels (Mytilus galloprovincialis) in Southern Italy
title_sort parasite load and strs genotyping of toxoplasma gondii isolates from mediterranean mussels (mytilus galloprovincialis) in southern italy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066981/
https://www.ncbi.nlm.nih.gov/pubmed/32210944
http://dx.doi.org/10.3389/fmicb.2020.00355
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