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Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice

CONTEXT: Oxidative imbalance-induced cognitive impairment is among the most urgent clinical concerns. Isoflurane has been demonstrated to impair cognitive function via an increase in oxidative stress. GSP has strong antioxidant capacities, suggesting potential cognitive benefits. OBJECTIVE: This stu...

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Autores principales: Gong, Xiangdan, Xu, Lizhi, Fang, Xin, Zhao, Xin, Du, Ying, Wu, Hao, Qian, Yue, Ma, Zhengliang, Xia, Tianjiao, Gu, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067175/
https://www.ncbi.nlm.nih.gov/pubmed/32114864
http://dx.doi.org/10.1080/13880209.2020.1730913
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author Gong, Xiangdan
Xu, Lizhi
Fang, Xin
Zhao, Xin
Du, Ying
Wu, Hao
Qian, Yue
Ma, Zhengliang
Xia, Tianjiao
Gu, Xiaoping
author_facet Gong, Xiangdan
Xu, Lizhi
Fang, Xin
Zhao, Xin
Du, Ying
Wu, Hao
Qian, Yue
Ma, Zhengliang
Xia, Tianjiao
Gu, Xiaoping
author_sort Gong, Xiangdan
collection PubMed
description CONTEXT: Oxidative imbalance-induced cognitive impairment is among the most urgent clinical concerns. Isoflurane has been demonstrated to impair cognitive function via an increase in oxidative stress. GSP has strong antioxidant capacities, suggesting potential cognitive benefits. OBJECTIVE: This study investigates whether GSP pre-treatment can alleviate isoflurane-induced cognitive dysfunction in mice. MATERIALS AND METHODS: C57BL/6J mice were pre-treated with either GSP 25–100 mg/kg/d for seven days or GSP 100–400 mg/kg as a single dose before the 6 h isoflurane anaesthesia. Cognitive functioning was examined using the fear conditioning tests. The levels of SOD, p-NR2B and p-CREB in the hippocampus were also analysed. RESULTS: Pre-treatment with either a dose of GSP 50 mg/kg/d for seven days or a single dose of GSP 200 mg/kg significantly increased the % freezing time in contextual tests on the 1st (72.18 ± 12.39% vs. 37.60 ± 8.93%; 78.27 ± 8.46% vs. 52.72 ± 2.64%), 3rd (93.80 ± 7.62% vs. 52.94 ± 14.10%; 87.65 ± 10.86% vs. 52.89 ± 1.73%) and 7th (91.36 ± 5.31% vs. 64.09 ± 14.46%; 93.78 ± 3.92% vs. 79.17 ± 1.79%) day after anaesthesia. In the hippocampus of mice exposed to isoflurane, GSP 200 mg/kg increased the total SOD activity on the 1st and 3rd day and reversed the decreased activity of the NR2B/CREB pathway. DISCUSSION AND CONCLUSIONS: These findings suggest that GSP improves isoflurane-induced cognitive dysfunction by protecting against perturbing antioxidant enzyme activities and NR2B/CREB pathway. Therefore, GSP may possess a potential prophylactic role in isoflurane-induced and other oxidative stress-related cognitive decline.
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spelling pubmed-70671752020-03-19 Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice Gong, Xiangdan Xu, Lizhi Fang, Xin Zhao, Xin Du, Ying Wu, Hao Qian, Yue Ma, Zhengliang Xia, Tianjiao Gu, Xiaoping Pharm Biol Research Article CONTEXT: Oxidative imbalance-induced cognitive impairment is among the most urgent clinical concerns. Isoflurane has been demonstrated to impair cognitive function via an increase in oxidative stress. GSP has strong antioxidant capacities, suggesting potential cognitive benefits. OBJECTIVE: This study investigates whether GSP pre-treatment can alleviate isoflurane-induced cognitive dysfunction in mice. MATERIALS AND METHODS: C57BL/6J mice were pre-treated with either GSP 25–100 mg/kg/d for seven days or GSP 100–400 mg/kg as a single dose before the 6 h isoflurane anaesthesia. Cognitive functioning was examined using the fear conditioning tests. The levels of SOD, p-NR2B and p-CREB in the hippocampus were also analysed. RESULTS: Pre-treatment with either a dose of GSP 50 mg/kg/d for seven days or a single dose of GSP 200 mg/kg significantly increased the % freezing time in contextual tests on the 1st (72.18 ± 12.39% vs. 37.60 ± 8.93%; 78.27 ± 8.46% vs. 52.72 ± 2.64%), 3rd (93.80 ± 7.62% vs. 52.94 ± 14.10%; 87.65 ± 10.86% vs. 52.89 ± 1.73%) and 7th (91.36 ± 5.31% vs. 64.09 ± 14.46%; 93.78 ± 3.92% vs. 79.17 ± 1.79%) day after anaesthesia. In the hippocampus of mice exposed to isoflurane, GSP 200 mg/kg increased the total SOD activity on the 1st and 3rd day and reversed the decreased activity of the NR2B/CREB pathway. DISCUSSION AND CONCLUSIONS: These findings suggest that GSP improves isoflurane-induced cognitive dysfunction by protecting against perturbing antioxidant enzyme activities and NR2B/CREB pathway. Therefore, GSP may possess a potential prophylactic role in isoflurane-induced and other oxidative stress-related cognitive decline. Taylor & Francis 2020-03-02 /pmc/articles/PMC7067175/ /pubmed/32114864 http://dx.doi.org/10.1080/13880209.2020.1730913 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gong, Xiangdan
Xu, Lizhi
Fang, Xin
Zhao, Xin
Du, Ying
Wu, Hao
Qian, Yue
Ma, Zhengliang
Xia, Tianjiao
Gu, Xiaoping
Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title_full Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title_fullStr Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title_full_unstemmed Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title_short Protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
title_sort protective effects of grape seed procyanidin on isoflurane-induced cognitive impairment in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067175/
https://www.ncbi.nlm.nih.gov/pubmed/32114864
http://dx.doi.org/10.1080/13880209.2020.1730913
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