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Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke

Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral...

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Autores principales: Ma, Junqiang, Ma, Yonglie, Shuaib, Ashfaq, Winship, Ian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067739/
https://www.ncbi.nlm.nih.gov/pubmed/31203565
http://dx.doi.org/10.1007/s12975-019-00710-1
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author Ma, Junqiang
Ma, Yonglie
Shuaib, Ashfaq
Winship, Ian R.
author_facet Ma, Junqiang
Ma, Yonglie
Shuaib, Ashfaq
Winship, Ian R.
author_sort Ma, Junqiang
collection PubMed
description Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral ischemia may vary with age but have not been extensively studied. Here, laser speckle contrast imaging (LSCI) and two-photon laser scanning microscopy (TPLSM) were combined to monitor cerebral pial collaterals between the anterior cerebral artery (ACA) and the middle cerebral artery (MCA) in young adult and aged male Sprague Dawley rats during distal middle cerebral artery occlusion (dMCAo). Histological analysis showed that aged rats had significantly greater volumes of ischemic damage than young rats. LSCI showed that cerebral collateral perfusion declined over time after stroke in aged and young rats, and that this decline was significantly greater in aged rats. TPLSM demonstrated that pial arterioles narrowed faster after dMCAo in aged rats compared to young adult rats. Notably, while arteriole vessel narrowing was comparable 4.5 h after ischemic onset in aged and young adult rats, red blood cell velocity was stable in young adults but declined over time in aged rats. Overall, red blood cell flux through pial arterioles was significantly reduced at all time-points after 90 min post-dMCAo in aged rats relative to young adult rats. Thus, collateral failure is more severe in aged rats with significantly impaired pial collateral dynamics (reduced diameter, red blood cell velocity, and red blood cell flux) relative to young adult rats.
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spelling pubmed-70677392020-03-23 Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke Ma, Junqiang Ma, Yonglie Shuaib, Ashfaq Winship, Ian R. Transl Stroke Res Original Article Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral ischemia may vary with age but have not been extensively studied. Here, laser speckle contrast imaging (LSCI) and two-photon laser scanning microscopy (TPLSM) were combined to monitor cerebral pial collaterals between the anterior cerebral artery (ACA) and the middle cerebral artery (MCA) in young adult and aged male Sprague Dawley rats during distal middle cerebral artery occlusion (dMCAo). Histological analysis showed that aged rats had significantly greater volumes of ischemic damage than young rats. LSCI showed that cerebral collateral perfusion declined over time after stroke in aged and young rats, and that this decline was significantly greater in aged rats. TPLSM demonstrated that pial arterioles narrowed faster after dMCAo in aged rats compared to young adult rats. Notably, while arteriole vessel narrowing was comparable 4.5 h after ischemic onset in aged and young adult rats, red blood cell velocity was stable in young adults but declined over time in aged rats. Overall, red blood cell flux through pial arterioles was significantly reduced at all time-points after 90 min post-dMCAo in aged rats relative to young adult rats. Thus, collateral failure is more severe in aged rats with significantly impaired pial collateral dynamics (reduced diameter, red blood cell velocity, and red blood cell flux) relative to young adult rats. Springer US 2019-06-15 2020 /pmc/articles/PMC7067739/ /pubmed/31203565 http://dx.doi.org/10.1007/s12975-019-00710-1 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Ma, Junqiang
Ma, Yonglie
Shuaib, Ashfaq
Winship, Ian R.
Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title_full Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title_fullStr Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title_full_unstemmed Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title_short Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
title_sort impaired collateral flow in pial arterioles of aged rats during ischemic stroke
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067739/
https://www.ncbi.nlm.nih.gov/pubmed/31203565
http://dx.doi.org/10.1007/s12975-019-00710-1
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