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Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins
The controllable production of microparticles with complex geometries is useful for a variety of applications in materials science and bioengineering. The formation of intricate microarchitectures typically requires sophisticated fabrication techniques such as flow lithography or multiple-emulsion m...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067844/ https://www.ncbi.nlm.nih.gov/pubmed/32165622 http://dx.doi.org/10.1038/s41467-020-15128-9 |
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author | Roberts, Stefan Miao, Vincent Costa, Simone Simon, Joseph Kelly, Garrett Shah, Tejank Zauscher, Stefan Chilkoti, Ashutosh |
author_facet | Roberts, Stefan Miao, Vincent Costa, Simone Simon, Joseph Kelly, Garrett Shah, Tejank Zauscher, Stefan Chilkoti, Ashutosh |
author_sort | Roberts, Stefan |
collection | PubMed |
description | The controllable production of microparticles with complex geometries is useful for a variety of applications in materials science and bioengineering. The formation of intricate microarchitectures typically requires sophisticated fabrication techniques such as flow lithography or multiple-emulsion microfluidics. By harnessing the molecular interactions of a set of artificial intrinsically disordered proteins (IDPs), we have created complex microparticle geometries, including porous particles, core-shell and hollow shell structures, and a unique ‘fruits-on-a-vine’ arrangement, by exploiting the metastable region of the phase diagram of thermally responsive IDPs within microdroplets. Through multi-site unnatural amino acid (UAA) incorporation, these protein microparticles can also be photo-crosslinked and stably extracted to an all-aqueous environment. This work expands the functional utility of artificial IDPs as well as the available microarchitectures of this class of biocompatible IDPs, with potential applications in drug delivery and tissue engineering. |
format | Online Article Text |
id | pubmed-7067844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70678442020-03-18 Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins Roberts, Stefan Miao, Vincent Costa, Simone Simon, Joseph Kelly, Garrett Shah, Tejank Zauscher, Stefan Chilkoti, Ashutosh Nat Commun Article The controllable production of microparticles with complex geometries is useful for a variety of applications in materials science and bioengineering. The formation of intricate microarchitectures typically requires sophisticated fabrication techniques such as flow lithography or multiple-emulsion microfluidics. By harnessing the molecular interactions of a set of artificial intrinsically disordered proteins (IDPs), we have created complex microparticle geometries, including porous particles, core-shell and hollow shell structures, and a unique ‘fruits-on-a-vine’ arrangement, by exploiting the metastable region of the phase diagram of thermally responsive IDPs within microdroplets. Through multi-site unnatural amino acid (UAA) incorporation, these protein microparticles can also be photo-crosslinked and stably extracted to an all-aqueous environment. This work expands the functional utility of artificial IDPs as well as the available microarchitectures of this class of biocompatible IDPs, with potential applications in drug delivery and tissue engineering. Nature Publishing Group UK 2020-03-12 /pmc/articles/PMC7067844/ /pubmed/32165622 http://dx.doi.org/10.1038/s41467-020-15128-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Roberts, Stefan Miao, Vincent Costa, Simone Simon, Joseph Kelly, Garrett Shah, Tejank Zauscher, Stefan Chilkoti, Ashutosh Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title | Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title_full | Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title_fullStr | Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title_full_unstemmed | Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title_short | Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
title_sort | complex microparticle architectures from stimuli-responsive intrinsically disordered proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067844/ https://www.ncbi.nlm.nih.gov/pubmed/32165622 http://dx.doi.org/10.1038/s41467-020-15128-9 |
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