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Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation

In addition to their role in regulating transport across the nuclear envelope, increasing evidence suggests nuclear pore complexes (NPCs) function in regulating gene expression. For example, the induction of certain genes (e.g., yeast INO1) is accompanied by their movement from the nuclear interior...

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Autores principales: Saik, Natasha O., Park, Nogi, Ptak, Christopher, Adames, Neil, Aitchison, John D., Wozniak, Richard W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067905/
https://www.ncbi.nlm.nih.gov/pubmed/32211027
http://dx.doi.org/10.3389/fgene.2020.00174
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author Saik, Natasha O.
Park, Nogi
Ptak, Christopher
Adames, Neil
Aitchison, John D.
Wozniak, Richard W.
author_facet Saik, Natasha O.
Park, Nogi
Ptak, Christopher
Adames, Neil
Aitchison, John D.
Wozniak, Richard W.
author_sort Saik, Natasha O.
collection PubMed
description In addition to their role in regulating transport across the nuclear envelope, increasing evidence suggests nuclear pore complexes (NPCs) function in regulating gene expression. For example, the induction of certain genes (e.g., yeast INO1) is accompanied by their movement from the nuclear interior to NPCs. As sumoylation has been linked to the regulation of chromatin spatial organization and transcriptional activity, we investigated the role of sumoylation in the expression and NPC recruitment of the INO1 gene. We observed that induction of INO1 is accompanied by both increased and decreased sumoylation of proteins associated with specific regions along the INO1 locus. Furthermore, we show that the E3 ligase Siz2/Nfi1 is required for targeting the INO1 locus to the NPC where it interacts with the SUMO isopeptidase Ulp1. Our data suggest that this interaction is required for both the association of INO1 with the NPC and for its normal expression. These results imply that sumoylation is a key regulator of INO1 targeting to the NPC, and a cycle of sumoylation and NPC-associated desumoylation events contribute to the regulation of INO1 expression.
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spelling pubmed-70679052020-03-24 Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation Saik, Natasha O. Park, Nogi Ptak, Christopher Adames, Neil Aitchison, John D. Wozniak, Richard W. Front Genet Genetics In addition to their role in regulating transport across the nuclear envelope, increasing evidence suggests nuclear pore complexes (NPCs) function in regulating gene expression. For example, the induction of certain genes (e.g., yeast INO1) is accompanied by their movement from the nuclear interior to NPCs. As sumoylation has been linked to the regulation of chromatin spatial organization and transcriptional activity, we investigated the role of sumoylation in the expression and NPC recruitment of the INO1 gene. We observed that induction of INO1 is accompanied by both increased and decreased sumoylation of proteins associated with specific regions along the INO1 locus. Furthermore, we show that the E3 ligase Siz2/Nfi1 is required for targeting the INO1 locus to the NPC where it interacts with the SUMO isopeptidase Ulp1. Our data suggest that this interaction is required for both the association of INO1 with the NPC and for its normal expression. These results imply that sumoylation is a key regulator of INO1 targeting to the NPC, and a cycle of sumoylation and NPC-associated desumoylation events contribute to the regulation of INO1 expression. Frontiers Media S.A. 2020-03-06 /pmc/articles/PMC7067905/ /pubmed/32211027 http://dx.doi.org/10.3389/fgene.2020.00174 Text en Copyright © 2020 Saik, Park, Ptak, Adames, Aitchison and Wozniak. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Saik, Natasha O.
Park, Nogi
Ptak, Christopher
Adames, Neil
Aitchison, John D.
Wozniak, Richard W.
Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title_full Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title_fullStr Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title_full_unstemmed Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title_short Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation
title_sort recruitment of an activated gene to the yeast nuclear pore complex requires sumoylation
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067905/
https://www.ncbi.nlm.nih.gov/pubmed/32211027
http://dx.doi.org/10.3389/fgene.2020.00174
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