Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells

We investigated the expression of OCC-1 at mRNA level during retinoic acid (RA) induced differentiation of mouse embryonic carcinoma P19 pluripotent cancer cells by quantitative real time PCR (qPCR). By employing four-fold serial dilutions of P19 cDNA, standard curves were generated for the referenc...

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Detalles Bibliográficos
Autores principales: Hosseininia, Zahra, Soltanian, Sara, Mahdavi-Shahri, Naser, Dehghani, Hesam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068043/
https://www.ncbi.nlm.nih.gov/pubmed/32190725
http://dx.doi.org/10.1016/j.dib.2020.105367
Descripción
Sumario:We investigated the expression of OCC-1 at mRNA level during retinoic acid (RA) induced differentiation of mouse embryonic carcinoma P19 pluripotent cancer cells by quantitative real time PCR (qPCR). By employing four-fold serial dilutions of P19 cDNA, standard curves were generated for the reference gene (L37) and the gene of interest (OCC-1). PCR efficiencies for L37 and OCC-1 were calculated. Since the amplification efficiencies of these two genes were unequal, the standard curve method was used for the relative quantification of OCC-1. Data analysis revealed that the expression of OCC-1 was reduced by about 69% after 4-day treatment with RA, when significant down-regulation of key pluripotency factors, including OCT4 and Nanog was observed [1].

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