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Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†)
Identification of reliable characteristics of follicle quality and developmental competence has been pursued in numerous studies, but with inconsistent outcomes. Here, we aimed to identify these characteristics by analysis of the follicular fluid (FF) steroid profile in relation to cumulus-oocyte co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068110/ https://www.ncbi.nlm.nih.gov/pubmed/31786607 http://dx.doi.org/10.1093/biolre/ioz217 |
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author | Costermans, N G J Soede, N M van Tricht, F Blokland, M Kemp, B Keijer, J Teerds, K J |
author_facet | Costermans, N G J Soede, N M van Tricht, F Blokland, M Kemp, B Keijer, J Teerds, K J |
author_sort | Costermans, N G J |
collection | PubMed |
description | Identification of reliable characteristics of follicle quality and developmental competence has been pursued in numerous studies, but with inconsistent outcomes. Here, we aimed to identify these characteristics by analysis of the follicular fluid (FF) steroid profile in relation to cumulus-oocyte complex (COC) morphology and follicle size, followed by molecular substantiation. Multiparous sows at weaning were used to facilitate analysis at the start of the follicular phase of the oestrus cycle. Sows with a higher average follicle size (≥5 mm vs. < 5 mm) had a higher follicular fluid β-estradiol concentration, but did not differ in other measured steroids. Sows with high compared to low percentage high-quality COCs (<70% vs. ≥70% high-quality) had follicular fluid with a higher concentration of β-estradiol, 19-norandrostenedione, progesterone, and α-testosterone, while the concentration of cortisol was lower. Transcriptome analysis of granulosa cells of healthy follicles of sows with a high percentage high-quality COCs showed higher abundance of transcripts involved in ovarian steroidogenesis (e.g., CYP19A2 and 3, POR, VEGFA) and growth (IGF1) and differential abundance of transcripts involved in granulosa cell apoptosis (e.g., GADD45A, INHBB). Differences in aromatase transcript abundance (CYP19A1, 2 and 3) were confirmed at the protein level. In addition, sows with a high percentage high-quality COCs lost less weight during lactation and had higher plasma IGF1 concentration at weaning, which may have affected COC quality. To the best of our knowledge, this study is also the first to report the relation between FF steroid profile and COC quality. |
format | Online Article Text |
id | pubmed-7068110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70681102020-03-18 Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) Costermans, N G J Soede, N M van Tricht, F Blokland, M Kemp, B Keijer, J Teerds, K J Biol Reprod Research Article Identification of reliable characteristics of follicle quality and developmental competence has been pursued in numerous studies, but with inconsistent outcomes. Here, we aimed to identify these characteristics by analysis of the follicular fluid (FF) steroid profile in relation to cumulus-oocyte complex (COC) morphology and follicle size, followed by molecular substantiation. Multiparous sows at weaning were used to facilitate analysis at the start of the follicular phase of the oestrus cycle. Sows with a higher average follicle size (≥5 mm vs. < 5 mm) had a higher follicular fluid β-estradiol concentration, but did not differ in other measured steroids. Sows with high compared to low percentage high-quality COCs (<70% vs. ≥70% high-quality) had follicular fluid with a higher concentration of β-estradiol, 19-norandrostenedione, progesterone, and α-testosterone, while the concentration of cortisol was lower. Transcriptome analysis of granulosa cells of healthy follicles of sows with a high percentage high-quality COCs showed higher abundance of transcripts involved in ovarian steroidogenesis (e.g., CYP19A2 and 3, POR, VEGFA) and growth (IGF1) and differential abundance of transcripts involved in granulosa cell apoptosis (e.g., GADD45A, INHBB). Differences in aromatase transcript abundance (CYP19A1, 2 and 3) were confirmed at the protein level. In addition, sows with a high percentage high-quality COCs lost less weight during lactation and had higher plasma IGF1 concentration at weaning, which may have affected COC quality. To the best of our knowledge, this study is also the first to report the relation between FF steroid profile and COC quality. Oxford University Press 2020-03 2019-11-30 /pmc/articles/PMC7068110/ /pubmed/31786607 http://dx.doi.org/10.1093/biolre/ioz217 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Costermans, N G J Soede, N M van Tricht, F Blokland, M Kemp, B Keijer, J Teerds, K J Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title | Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title_full | Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title_fullStr | Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title_full_unstemmed | Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title_short | Follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
title_sort | follicular fluid steroid profile in sows: relationship to follicle size and oocyte quality(†) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068110/ https://www.ncbi.nlm.nih.gov/pubmed/31786607 http://dx.doi.org/10.1093/biolre/ioz217 |
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