Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system

Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the ‘ope...

Descripción completa

Detalles Bibliográficos
Autores principales: Pfefferle, Susanne, Reucher, Svenja, Nörz, Dominic, Lütgehetmann, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068162/
https://www.ncbi.nlm.nih.gov/pubmed/32156329
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000152
_version_ 1783505518744043520
author Pfefferle, Susanne
Reucher, Svenja
Nörz, Dominic
Lütgehetmann, Marc
author_facet Pfefferle, Susanne
Reucher, Svenja
Nörz, Dominic
Lütgehetmann, Marc
author_sort Pfefferle, Susanne
collection PubMed
description Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the ‘open channel’ for integration of a laboratory-developed assay. We observed good analytical performance in clinical specimens. The fully automated workflow enables high-throughput testing with minimal hands-on time, while offering fast and reliable results.
format Online
Article
Text
id pubmed-7068162
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher European Centre for Disease Prevention and Control (ECDC)
record_format MEDLINE/PubMed
spelling pubmed-70681622020-03-18 Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system Pfefferle, Susanne Reucher, Svenja Nörz, Dominic Lütgehetmann, Marc Euro Surveill Rapid Communication Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the ‘open channel’ for integration of a laboratory-developed assay. We observed good analytical performance in clinical specimens. The fully automated workflow enables high-throughput testing with minimal hands-on time, while offering fast and reliable results. European Centre for Disease Prevention and Control (ECDC) 2020-03-05 /pmc/articles/PMC7068162/ /pubmed/32156329 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000152 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Pfefferle, Susanne
Reucher, Svenja
Nörz, Dominic
Lütgehetmann, Marc
Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title_full Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title_fullStr Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title_full_unstemmed Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title_short Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
title_sort evaluation of a quantitative rt-pcr assay for the detection of the emerging coronavirus sars-cov-2 using a high throughput system
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068162/
https://www.ncbi.nlm.nih.gov/pubmed/32156329
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000152
work_keys_str_mv AT pfefferlesusanne evaluationofaquantitativertpcrassayforthedetectionoftheemergingcoronavirussarscov2usingahighthroughputsystem
AT reuchersvenja evaluationofaquantitativertpcrassayforthedetectionoftheemergingcoronavirussarscov2usingahighthroughputsystem
AT norzdominic evaluationofaquantitativertpcrassayforthedetectionoftheemergingcoronavirussarscov2usingahighthroughputsystem
AT lutgehetmannmarc evaluationofaquantitativertpcrassayforthedetectionoftheemergingcoronavirussarscov2usingahighthroughputsystem

Ejemplares similares