Cargando…

Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system

Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the ‘ope...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pfefferle, Susanne, Reucher, Svenja, Nörz, Dominic, Lütgehetmann, Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	European Centre for Disease Prevention and Control (ECDC) 2020
Materias:	Rapid Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068162/ https://www.ncbi.nlm.nih.gov/pubmed/32156329 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000152

Ejemplares similares

Clinical evaluation of a fully automated, laboratory-developed multiplex RT-PCR assay integrating dual-target SARS-CoV-2 and influenza A/B detection on a high-throughput platform
por: Nörz, Dominik, et al.
Publicado: (2021)

Clinical evaluation of a SARS-CoV-2 RT-PCR assay on a fully automated system for rapid on-demand testing in the hospital setting
por: Nörz, Dominik, et al.
Publicado: (2020)

Pushing beyond specifications: Evaluation of linearity and clinical performance of the cobas 6800/8800 SARS-CoV-2 RT-PCR assay for reliable quantification in blood and other materials outside recommendations
por: Nörz, Dominik, et al.
Publicado: (2020)

Modifying a Diagnostic SARS-CoV-2 Spike PCR to Turn a Del69/70 Dropout into a Discriminatory On-Target Assay
por: Nörz, Dominik, et al.
Publicado: (2021)

Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with built-in screening functionality for del-HV69/70- and N501Y variants such as B.1.1.7
por: Nörz, Dominik, et al.
Publicado: (2021)

Clinical establishment of a laboratory developed quantitative HDV PCR assay on the cobas6800 high-throughput system
por: Pflüger, Lisa Sophie, et al.
Publicado: (2021)

Handling and accuracy of four rapid antigen tests for the diagnosis of SARS-CoV-2 compared to RT-qPCR
por: Olearo, Flaminia, et al.
Publicado: (2021)

Evidence of surface contamination in hospital rooms occupied by patients infected with monkeypox, Germany, June 2022
por: Nörz, Dominik, et al.
Publicado: (2022)

Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020
por: Muenchhoff, Maximilian, et al.
Publicado: (2020)

Clinical Evaluation of a Fully-Automated High-Throughput Multiplex Screening-Assay to Detect and Differentiate the SARS-CoV-2 B.1.1.529 (Omicron) and B.1.617.2 (Delta) Lineage Variants
por: Nörz, Dominik, et al.
Publicado: (2022)

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection
por: Nörz, Dominik, et al.
Publicado: (2022)

Rapid Automated Screening for SARS-CoV-2 B.1.617 Lineage Variants (Delta/Kappa) through a Versatile Toolset of qPCR-Based SNP Detection
por: Nörz, Dominik, et al.
Publicado: (2021)

Complete Genome Sequence of a SARS-CoV-2 Strain Isolated in Northern Germany
por: Pfefferle, Susanne, et al.
Publicado: (2020)

One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses
por: Rački, Nejc, et al.
Publicado: (2013)

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
por: Vijgen, Leen, et al.
Publicado: (2007)

Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
por: Mo, Jongseo, et al.
Publicado: (2022)

Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19
por: Casagrande, Maria, et al.
Publicado: (2022)

Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
por: Carroll, Anne, et al.
Publicado: (2021)

Quantitative RT-PCR assay for high-throughput screening (HTS) of drugs against the growth of Cryptosporidium parvum in vitro
por: Zhang, Haili, et al.
Publicado: (2015)

Viral Dynamics of SARS-CoV-2 in Critically Ill Allogeneic Hematopoietic Stem Cell Transplant Recipients and Immunocompetent Patients with COVID-19
por: Roedl, Kevin, et al.
Publicado: (2021)

Data-driven normalization strategies for high-throughput quantitative RT-PCR
por: Mar, Jessica C, et al.
Publicado: (2009)

Postmortem Stability of SARS-CoV-2 in Nasopharyngeal Mucosa
por: Heinrich, Fabian, et al.
Publicado: (2021)

An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes
por: Sun, Junfeng, et al.
Publicado: (2015)

Development of a high-throughput colorimetric Zika virus infection assay
por: Müller, Janis A., et al.
Publicado: (2017)

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020
por: Singanayagam, Anika, et al.
Publicado: (2020)

SARS Coronavirus-2 variant tracing within the first Coronavirus Disease 19 clusters in northern Germany
por: Pfefferle, Susanne, et al.
Publicado: (2021)

SARS-CoV-2 Blood RNA Load Predicts Outcome in Critically Ill COVID-19 Patients
por: Heinrich, Fabian, et al.
Publicado: (2021)

Infectivity of SARS-CoV-2 on Inanimate Surfaces: Don’t Trust Ct Value
por: Knobloch, Johannes K., et al.
Publicado: (2022)

Evaluation of a laboratory-based high-throughput SARS-CoV-2 antigen assay for non-COVID-19 patient screening at hospital admission
por: Häuser, Friederike, et al.
Publicado: (2021)

Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis
por: Soma, Takehisa
Publicado: (2015)

A RT-PCR assay for the detection of coronaviruses from four genera
por: Xiu, Leshan, et al.
Publicado: (2020)

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
por: Renn, Lynnsey A., et al.
Publicado: (2015)

Multicenter Evaluation of a Fully Automated High-Throughput SARS-CoV-2 Antigen Immunoassay
por: Nörz, Dominik, et al.
Publicado: (2021)

Pitfalls in SARS‐CoV‐2 PCR diagnostics
por: Wernike, Kerstin, et al.
Publicado: (2020)

A species-specific triplex PCR assay for authentication of Galli Gigerii Endothelium Corneum
por: Zhang, Yuli, et al.
Publicado: (2021)

Potential added value of a RT-qPCR method of SOX 11 expression, in the context of a multidisciplinary diagnostic assessment of B cell malignancies
por: Magne, Julien, et al.
Publicado: (2018)

Clinical efficacy and in vitro neutralization capacity of monoclonal antibodies for severe acute respiratory syndrome coronavirus 2 delta and omicron variants
por: Brehm, Thomas Theo, et al.
Publicado: (2022)

RT-PCR testing should be performed prior to elective orthopaedic surgery during the COVID-19 pandemic
por: Nakai, Tsuyoshi, et al.
Publicado: (2021)

Enhanced throughput of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR panel by assay multiplexing and specimen pooling
por: Lu, Xiaoyan, et al.
Publicado: (2021)

A high-throughput assay for quantitative measurement of PCR errors
por: Shagin, Dmitriy A., et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_umsedhumv2vqn19bfanrhnkkms