Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing...

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Detalles Bibliográficos
Autores principales: Konrad, Regina, Eberle, Ute, Dangel, Alexandra, Treis, Bianca, Berger, Anja, Bengs, Katja, Fingerle, Volker, Liebl, Bernhard, Ackermann, Nikolaus, Sing, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068163/
https://www.ncbi.nlm.nih.gov/pubmed/32156330
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000173
Descripción
Sumario:The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.

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