Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing...

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Autores principales: Konrad, Regina, Eberle, Ute, Dangel, Alexandra, Treis, Bianca, Berger, Anja, Bengs, Katja, Fingerle, Volker, Liebl, Bernhard, Ackermann, Nikolaus, Sing, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068163/
https://www.ncbi.nlm.nih.gov/pubmed/32156330
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000173
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author Konrad, Regina
Eberle, Ute
Dangel, Alexandra
Treis, Bianca
Berger, Anja
Bengs, Katja
Fingerle, Volker
Liebl, Bernhard
Ackermann, Nikolaus
Sing, Andreas
author_facet Konrad, Regina
Eberle, Ute
Dangel, Alexandra
Treis, Bianca
Berger, Anja
Bengs, Katja
Fingerle, Volker
Liebl, Bernhard
Ackermann, Nikolaus
Sing, Andreas
author_sort Konrad, Regina
collection PubMed
description The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
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spelling pubmed-70681632020-03-18 Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020 Konrad, Regina Eberle, Ute Dangel, Alexandra Treis, Bianca Berger, Anja Bengs, Katja Fingerle, Volker Liebl, Bernhard Ackermann, Nikolaus Sing, Andreas Euro Surveill Rapid Communication The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well. European Centre for Disease Prevention and Control (ECDC) 2020-03-05 /pmc/articles/PMC7068163/ /pubmed/32156330 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000173 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Konrad, Regina
Eberle, Ute
Dangel, Alexandra
Treis, Bianca
Berger, Anja
Bengs, Katja
Fingerle, Volker
Liebl, Bernhard
Ackermann, Nikolaus
Sing, Andreas
Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title_full Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title_fullStr Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title_full_unstemmed Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title_short Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
title_sort rapid establishment of laboratory diagnostics for the novel coronavirus sars-cov-2 in bavaria, germany, february 2020
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068163/
https://www.ncbi.nlm.nih.gov/pubmed/32156330
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.9.2000173
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