Cargando…
Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata
It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, ost...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068328/ https://www.ncbi.nlm.nih.gov/pubmed/32098178 http://dx.doi.org/10.3390/ijerph17041391 |
_version_ | 1783505554733268992 |
---|---|
author | Zanaty, Mohamed I. Sawada, Niina Kitani, Yoichiro Nassar, Hossam F. Mahmoud, Hamada M. Hayakawa, Kazuichi Sekiguchi, Toshio Ogiso, Shouzo Tabuchi, Yoshiaki Urata, Makoto Matsubara, Hajime Takeuchi, Yutaka Hattori, Atsuhiko Srivastav, Ajai K. Amornsakun, Thumronk Suzuki, Nobuo |
author_facet | Zanaty, Mohamed I. Sawada, Niina Kitani, Yoichiro Nassar, Hossam F. Mahmoud, Hamada M. Hayakawa, Kazuichi Sekiguchi, Toshio Ogiso, Shouzo Tabuchi, Yoshiaki Urata, Makoto Matsubara, Hajime Takeuchi, Yutaka Hattori, Atsuhiko Srivastav, Ajai K. Amornsakun, Thumronk Suzuki, Nobuo |
author_sort | Zanaty, Mohamed I. |
collection | PubMed |
description | It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution. |
format | Online Article Text |
id | pubmed-7068328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70683282020-03-19 Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata Zanaty, Mohamed I. Sawada, Niina Kitani, Yoichiro Nassar, Hossam F. Mahmoud, Hamada M. Hayakawa, Kazuichi Sekiguchi, Toshio Ogiso, Shouzo Tabuchi, Yoshiaki Urata, Makoto Matsubara, Hajime Takeuchi, Yutaka Hattori, Atsuhiko Srivastav, Ajai K. Amornsakun, Thumronk Suzuki, Nobuo Int J Environ Res Public Health Article It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution. MDPI 2020-02-21 2020-02 /pmc/articles/PMC7068328/ /pubmed/32098178 http://dx.doi.org/10.3390/ijerph17041391 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zanaty, Mohamed I. Sawada, Niina Kitani, Yoichiro Nassar, Hossam F. Mahmoud, Hamada M. Hayakawa, Kazuichi Sekiguchi, Toshio Ogiso, Shouzo Tabuchi, Yoshiaki Urata, Makoto Matsubara, Hajime Takeuchi, Yutaka Hattori, Atsuhiko Srivastav, Ajai K. Amornsakun, Thumronk Suzuki, Nobuo Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title | Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title_full | Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title_fullStr | Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title_full_unstemmed | Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title_short | Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata |
title_sort | influence of benz[a]anthracene on bone metabolism and on liver metabolism in nibbler fish, girella punctata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068328/ https://www.ncbi.nlm.nih.gov/pubmed/32098178 http://dx.doi.org/10.3390/ijerph17041391 |
work_keys_str_mv | AT zanatymohamedi influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT sawadaniina influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT kitaniyoichiro influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT nassarhossamf influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT mahmoudhamadam influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT hayakawakazuichi influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT sekiguchitoshio influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT ogisoshouzo influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT tabuchiyoshiaki influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT uratamakoto influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT matsubarahajime influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT takeuchiyutaka influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT hattoriatsuhiko influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT srivastavajaik influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT amornsakunthumronk influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata AT suzukinobuo influenceofbenzaanthraceneonbonemetabolismandonlivermetabolisminnibblerfishgirellapunctata |