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Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A

Polycyclic aromatic hydrocarbons (PAHs) are pollutants that exert harmful effects on marine invertebrates; however, the molecular mechanism underlying PAH action remains unclear. We investigated the effect of PAHs on the ascidian Ciona intestinalis type A (Ciona robusta). First, the influence of PAH...

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Autores principales: Sekiguchi, Toshio, Akitaya, Hiroshi, Nakayama, Satoshi, Yazawa, Takashi, Ogasawara, Michio, Suzuki, Nobuo, Hayakawa, Kazuichi, Wada, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068557/
https://www.ncbi.nlm.nih.gov/pubmed/32093017
http://dx.doi.org/10.3390/ijerph17041340
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author Sekiguchi, Toshio
Akitaya, Hiroshi
Nakayama, Satoshi
Yazawa, Takashi
Ogasawara, Michio
Suzuki, Nobuo
Hayakawa, Kazuichi
Wada, Shuichi
author_facet Sekiguchi, Toshio
Akitaya, Hiroshi
Nakayama, Satoshi
Yazawa, Takashi
Ogasawara, Michio
Suzuki, Nobuo
Hayakawa, Kazuichi
Wada, Shuichi
author_sort Sekiguchi, Toshio
collection PubMed
description Polycyclic aromatic hydrocarbons (PAHs) are pollutants that exert harmful effects on marine invertebrates; however, the molecular mechanism underlying PAH action remains unclear. We investigated the effect of PAHs on the ascidian Ciona intestinalis type A (Ciona robusta). First, the influence of PAHs on early Ciona development was evaluated. PAHs such as dibenzothiophene, fluorene, and phenanthrene resulted in formation of abnormal larvae. PAH treatment of swimming larva induced malformation in the form of tail regression. Additionally, we observed the Ciona aryl hydrocarbon receptor (Ci-AhR) mRNA expression in swimming larva, mid body axis rotation, and early juvenile stages. The time correlation between PAH action and AhR mRNA expression suggested that Ci-AhR could be associated with PAH metabolism. Lastly, we analyzed Ci-AhR mRNA localization in Ciona juveniles. Ci-AhR mRNA was localized in the digestive tract, dorsal tubercle, ganglion, and papillae of the branchial sac, suggesting that Ci-AhR is a candidate for an environmental pollutant sensor and performs a neural function. Our results provide basic knowledge on the biological function of Ci-AhR and PAH activity in marine invertebrates.
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spelling pubmed-70685572020-03-19 Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A Sekiguchi, Toshio Akitaya, Hiroshi Nakayama, Satoshi Yazawa, Takashi Ogasawara, Michio Suzuki, Nobuo Hayakawa, Kazuichi Wada, Shuichi Int J Environ Res Public Health Article Polycyclic aromatic hydrocarbons (PAHs) are pollutants that exert harmful effects on marine invertebrates; however, the molecular mechanism underlying PAH action remains unclear. We investigated the effect of PAHs on the ascidian Ciona intestinalis type A (Ciona robusta). First, the influence of PAHs on early Ciona development was evaluated. PAHs such as dibenzothiophene, fluorene, and phenanthrene resulted in formation of abnormal larvae. PAH treatment of swimming larva induced malformation in the form of tail regression. Additionally, we observed the Ciona aryl hydrocarbon receptor (Ci-AhR) mRNA expression in swimming larva, mid body axis rotation, and early juvenile stages. The time correlation between PAH action and AhR mRNA expression suggested that Ci-AhR could be associated with PAH metabolism. Lastly, we analyzed Ci-AhR mRNA localization in Ciona juveniles. Ci-AhR mRNA was localized in the digestive tract, dorsal tubercle, ganglion, and papillae of the branchial sac, suggesting that Ci-AhR is a candidate for an environmental pollutant sensor and performs a neural function. Our results provide basic knowledge on the biological function of Ci-AhR and PAH activity in marine invertebrates. MDPI 2020-02-19 2020-02 /pmc/articles/PMC7068557/ /pubmed/32093017 http://dx.doi.org/10.3390/ijerph17041340 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sekiguchi, Toshio
Akitaya, Hiroshi
Nakayama, Satoshi
Yazawa, Takashi
Ogasawara, Michio
Suzuki, Nobuo
Hayakawa, Kazuichi
Wada, Shuichi
Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title_full Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title_fullStr Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title_full_unstemmed Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title_short Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
title_sort effect of polycyclic aromatic hydrocarbons on development of the ascidian ciona intestinalis type a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068557/
https://www.ncbi.nlm.nih.gov/pubmed/32093017
http://dx.doi.org/10.3390/ijerph17041340
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