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Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with t...

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Autores principales: Panico, Alessandra, Grassi, Tiziana, Bagordo, Francesco, Idolo, Adele, Serio, Francesca, Tumolo, Maria Rosaria, De Giorgi, Mattia, Guido, Marcello, Tutino, Maria, De Donno, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068596/
https://www.ncbi.nlm.nih.gov/pubmed/32069990
http://dx.doi.org/10.3390/ijerph17041208
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author Panico, Alessandra
Grassi, Tiziana
Bagordo, Francesco
Idolo, Adele
Serio, Francesca
Tumolo, Maria Rosaria
De Giorgi, Mattia
Guido, Marcello
Tutino, Maria
De Donno, Antonella
author_facet Panico, Alessandra
Grassi, Tiziana
Bagordo, Francesco
Idolo, Adele
Serio, Francesca
Tumolo, Maria Rosaria
De Giorgi, Mattia
Guido, Marcello
Tutino, Maria
De Donno, Antonella
author_sort Panico, Alessandra
collection PubMed
description Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM(0.5)), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated.
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spelling pubmed-70685962020-03-19 Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy) Panico, Alessandra Grassi, Tiziana Bagordo, Francesco Idolo, Adele Serio, Francesca Tumolo, Maria Rosaria De Giorgi, Mattia Guido, Marcello Tutino, Maria De Donno, Antonella Int J Environ Res Public Health Article Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM(0.5)), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated. MDPI 2020-02-13 2020-02 /pmc/articles/PMC7068596/ /pubmed/32069990 http://dx.doi.org/10.3390/ijerph17041208 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panico, Alessandra
Grassi, Tiziana
Bagordo, Francesco
Idolo, Adele
Serio, Francesca
Tumolo, Maria Rosaria
De Giorgi, Mattia
Guido, Marcello
Tutino, Maria
De Donno, Antonella
Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title_full Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title_fullStr Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title_full_unstemmed Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title_short Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)
title_sort micronucleus frequency in exfoliated buccal cells of children living in an industrialized area of apulia (italy)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068596/
https://www.ncbi.nlm.nih.gov/pubmed/32069990
http://dx.doi.org/10.3390/ijerph17041208
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