Cargando…

RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis

Lung cancer is the most common cause of cancer-associated mortality in China with 85% of patients having non-small cell lung cancer (NSCLC). Identifying NSCLC driver genes and prognostic markers is critical to reducing these numbers. The studies of retinoblastoma binding protein 6 (RBBP6) performed...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Qiu-Shi, Wei, Shi-Rong, Xiao, Hua-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068609/
https://www.ncbi.nlm.nih.gov/pubmed/32218844
http://dx.doi.org/10.3892/ol.2020.11403
_version_ 1783505619462914048
author Wang, Qiu-Shi
Wei, Shi-Rong
Xiao, Hua-Liang
author_facet Wang, Qiu-Shi
Wei, Shi-Rong
Xiao, Hua-Liang
author_sort Wang, Qiu-Shi
collection PubMed
description Lung cancer is the most common cause of cancer-associated mortality in China with 85% of patients having non-small cell lung cancer (NSCLC). Identifying NSCLC driver genes and prognostic markers is critical to reducing these numbers. The studies of retinoblastoma binding protein 6 (RBBP6) performed on NSCLC is limited. The present study aimed to investigate the molecular function and the prognostic potential of RBBP6 in NSCLC using the A549 cell line and patient samples, respectively. The functional effect on cancer cell proliferation and prognostic value of RBBP6 were examined in vitro and in vivo using reverse transcription-quantitative PCR, immunofluorescence, immunohistochemistry (IHC) and xenograft implantation. The results demonstrated that RBBP6 mRNA expression was significantly higher in NSCLC tissues compared with in adjacent normal samples. When RBBP6 mRNA expression was interfered with using short hairpin RNA, A549 cell proliferation and xenograft tumor growth were reduced. Additionally, IHC and survival analysis demonstrated that patients with NSCLC with high expression levels of RBBP6 had a shorter median overall survival time compared with patients with low RBBP6 expression (31 vs. 51.5 months), and this was more prominent in stage I–II patients (43 vs. >67 months). High expression levels of RBBP6 indicated poor prognosis in patients with NSCLC. This may be due to the ability of RBBP6 to promote cancer cell proliferation. RBBP6 may be a potential prognostic biomarker and a therapeutic target for NSCLC.
format Online
Article
Text
id pubmed-7068609
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-70686092020-03-26 RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis Wang, Qiu-Shi Wei, Shi-Rong Xiao, Hua-Liang Oncol Lett Articles Lung cancer is the most common cause of cancer-associated mortality in China with 85% of patients having non-small cell lung cancer (NSCLC). Identifying NSCLC driver genes and prognostic markers is critical to reducing these numbers. The studies of retinoblastoma binding protein 6 (RBBP6) performed on NSCLC is limited. The present study aimed to investigate the molecular function and the prognostic potential of RBBP6 in NSCLC using the A549 cell line and patient samples, respectively. The functional effect on cancer cell proliferation and prognostic value of RBBP6 were examined in vitro and in vivo using reverse transcription-quantitative PCR, immunofluorescence, immunohistochemistry (IHC) and xenograft implantation. The results demonstrated that RBBP6 mRNA expression was significantly higher in NSCLC tissues compared with in adjacent normal samples. When RBBP6 mRNA expression was interfered with using short hairpin RNA, A549 cell proliferation and xenograft tumor growth were reduced. Additionally, IHC and survival analysis demonstrated that patients with NSCLC with high expression levels of RBBP6 had a shorter median overall survival time compared with patients with low RBBP6 expression (31 vs. 51.5 months), and this was more prominent in stage I–II patients (43 vs. >67 months). High expression levels of RBBP6 indicated poor prognosis in patients with NSCLC. This may be due to the ability of RBBP6 to promote cancer cell proliferation. RBBP6 may be a potential prognostic biomarker and a therapeutic target for NSCLC. D.A. Spandidos 2020-04 2020-02-17 /pmc/articles/PMC7068609/ /pubmed/32218844 http://dx.doi.org/10.3892/ol.2020.11403 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Qiu-Shi
Wei, Shi-Rong
Xiao, Hua-Liang
RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title_full RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title_fullStr RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title_full_unstemmed RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title_short RBBP6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
title_sort rbbp6 induces non-small cell lung cancer cell proliferation and high expression is associated with poor prognosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068609/
https://www.ncbi.nlm.nih.gov/pubmed/32218844
http://dx.doi.org/10.3892/ol.2020.11403
work_keys_str_mv AT wangqiushi rbbp6inducesnonsmallcelllungcancercellproliferationandhighexpressionisassociatedwithpoorprognosis
AT weishirong rbbp6inducesnonsmallcelllungcancercellproliferationandhighexpressionisassociatedwithpoorprognosis
AT xiaohualiang rbbp6inducesnonsmallcelllungcancercellproliferationandhighexpressionisassociatedwithpoorprognosis