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Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format
Background: Traditional lateral flow immunoassay (LFIA) based on 20-40 nm gold nanoparticles (AuNPs) as signal reporter always suffers from relatively low detection sensitivity due to its insufficient brightness, severely restricting its wide-ranging application in the detection of target analytes w...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069069/ https://www.ncbi.nlm.nih.gov/pubmed/32206119 http://dx.doi.org/10.7150/thno.42364 |
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author | Chen, Xirui Leng, Yuankui Hao, Liangwen Duan, Hong Yuan, Jing Zhang, Wenjing Huang, Xiaolin Xiong, Yonghua |
author_facet | Chen, Xirui Leng, Yuankui Hao, Liangwen Duan, Hong Yuan, Jing Zhang, Wenjing Huang, Xiaolin Xiong, Yonghua |
author_sort | Chen, Xirui |
collection | PubMed |
description | Background: Traditional lateral flow immunoassay (LFIA) based on 20-40 nm gold nanoparticles (AuNPs) as signal reporter always suffers from relatively low detection sensitivity due to its insufficient brightness, severely restricting its wide-ranging application in the detection of target analytes with trace concentration. Methods: To address this problem, the self-assembled colloidal gold superparticles (GSPs) were synthesized as an improved absorption-dominated labeling probe for improving the sensitivity of sandwich LFIA. Five kinds of GSPs with the size ranging from 100 nm to 400 nm were synthesized by embedding hydrophobic AuNPs of size 12 nm as building blocks into the polymer nanobeads. The as-prepared GSPs were suggested as novel labeling probes of LFIA. The effects of the size of assembled GSPs on the sensitivity of sandwich LFIA was assessed, and the detection performance of GSPs-LFIA was further compared with traditional AuNPs-LFIA. Results: The resultant GSPs showed extremely high light absorption but very low light scattering, which favor the absorption-dominated signal output in LFIA. Among them, the GSP(270)-LFIA (size 270 nm) exhibits the highest sensitivity for human chorionic gonadotropin and hepatitis B surface antigen detection in real serum sample, which are approximate 39.79- and 13.8-fold higher than that of traditional AuNP(40)-LFIA. Conclusions: The proposed research demonstrated that the current GSPs can provide an ultrasensitive and quantitative detection for disease biomarkers in real serum samples as promising reporters of sandwich LFIA platform. |
format | Online Article Text |
id | pubmed-7069069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-70690692020-03-23 Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format Chen, Xirui Leng, Yuankui Hao, Liangwen Duan, Hong Yuan, Jing Zhang, Wenjing Huang, Xiaolin Xiong, Yonghua Theranostics Research Paper Background: Traditional lateral flow immunoassay (LFIA) based on 20-40 nm gold nanoparticles (AuNPs) as signal reporter always suffers from relatively low detection sensitivity due to its insufficient brightness, severely restricting its wide-ranging application in the detection of target analytes with trace concentration. Methods: To address this problem, the self-assembled colloidal gold superparticles (GSPs) were synthesized as an improved absorption-dominated labeling probe for improving the sensitivity of sandwich LFIA. Five kinds of GSPs with the size ranging from 100 nm to 400 nm were synthesized by embedding hydrophobic AuNPs of size 12 nm as building blocks into the polymer nanobeads. The as-prepared GSPs were suggested as novel labeling probes of LFIA. The effects of the size of assembled GSPs on the sensitivity of sandwich LFIA was assessed, and the detection performance of GSPs-LFIA was further compared with traditional AuNPs-LFIA. Results: The resultant GSPs showed extremely high light absorption but very low light scattering, which favor the absorption-dominated signal output in LFIA. Among them, the GSP(270)-LFIA (size 270 nm) exhibits the highest sensitivity for human chorionic gonadotropin and hepatitis B surface antigen detection in real serum sample, which are approximate 39.79- and 13.8-fold higher than that of traditional AuNP(40)-LFIA. Conclusions: The proposed research demonstrated that the current GSPs can provide an ultrasensitive and quantitative detection for disease biomarkers in real serum samples as promising reporters of sandwich LFIA platform. Ivyspring International Publisher 2020-02-19 /pmc/articles/PMC7069069/ /pubmed/32206119 http://dx.doi.org/10.7150/thno.42364 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Chen, Xirui Leng, Yuankui Hao, Liangwen Duan, Hong Yuan, Jing Zhang, Wenjing Huang, Xiaolin Xiong, Yonghua Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title | Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title_full | Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title_fullStr | Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title_full_unstemmed | Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title_short | Self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
title_sort | self-assembled colloidal gold superparticles to enhance the sensitivity of lateral flow immunoassays with sandwich format |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069069/ https://www.ncbi.nlm.nih.gov/pubmed/32206119 http://dx.doi.org/10.7150/thno.42364 |
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