Cargando…

Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats

The present study aimed to assess the changes in circulating microRNA (miRNA) expression profiles associated with the potential osteoprotective effect of diosgenin (DIO) in ovariectomized (OVX) rats. Wistar rats (female) were subjected to a sham operation (SHAM group) or ovariectomy. OVX rats were t...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhiguo, Xiang, Lihua, Wang, Yuhan, Jiang, Yanhua, Cheng, Yin, Xiao, Gary Guishan, Ju, Dahong, Chen, Yanjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069125/
https://www.ncbi.nlm.nih.gov/pubmed/32210807
http://dx.doi.org/10.3389/fphar.2020.00207
_version_ 1783505717331755008
author Zhang, Zhiguo
Xiang, Lihua
Wang, Yuhan
Jiang, Yanhua
Cheng, Yin
Xiao, Gary Guishan
Ju, Dahong
Chen, Yanjing
author_facet Zhang, Zhiguo
Xiang, Lihua
Wang, Yuhan
Jiang, Yanhua
Cheng, Yin
Xiao, Gary Guishan
Ju, Dahong
Chen, Yanjing
author_sort Zhang, Zhiguo
collection PubMed
description The present study aimed to assess the changes in circulating microRNA (miRNA) expression profiles associated with the potential osteoprotective effect of diosgenin (DIO) in ovariectomized (OVX) rats. Wistar rats (female) were subjected to a sham operation (SHAM group) or ovariectomy. OVX rats were treated with DIO (DIO group) or vehicle (OVX group) for 12 weeks. Following treatment, the serum estradiol, bone turnover biomarker levels, and the microarchitecture of tibias were assayed. Based on miRNA microarray and qRT-PCR analyses, differentially expressed (DE) circulating miRNAs were identified between the OVX and SHAM groups (comparison A) and between the DIO and OVX groups (comparison B). Furthermore, putative target genes of shared DE miRNAs with opposite expression trends in the two comparisons were predicted by ingenuity pathway analysis (IPA). Finally, the expression levels of the putative target genes in serum and tibia were validated by qRT-PCR. The micro-CT results demonstrated that DIO had a substantial anti-osteopenic effect on the tibias of OVX rats. In total, we found 5 DE circulating miRNAs (four upregulated and one downregulated) in comparison A and 21 DE circulating miRNAs (15 upregulated and 6 downregulated) in comparison B. However, only one DE circulating miRNA (rno-miR-20a-5p) had opposite expression trends between the two comparisons. Including rno-miR-20a-5p, 7 of the 10 selected DE circulating miRNAs between the two comparisons passed qRT-PCR validation. Specifically, based on qRT-PCR validation, DIO upregulated the expression of rno-miR-20a-5p and downregulated that of three target genes (Tnf, Creb1, and Tgfbr2) of the “osteoclast differentiation” pathway in the tibias of OVX rats. Our results suggested that DIO could change the circulating miRNA profile of OVX rats and inhibited the downregulation of miR-20a-5p in serum and tibia. DIO might exert an anti-osteoclastogenic effect on OVX rats by upregulating the expression of miR-20a-5p in circulation and bone tissue.
format Online
Article
Text
id pubmed-7069125
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-70691252020-03-24 Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats Zhang, Zhiguo Xiang, Lihua Wang, Yuhan Jiang, Yanhua Cheng, Yin Xiao, Gary Guishan Ju, Dahong Chen, Yanjing Front Pharmacol Pharmacology The present study aimed to assess the changes in circulating microRNA (miRNA) expression profiles associated with the potential osteoprotective effect of diosgenin (DIO) in ovariectomized (OVX) rats. Wistar rats (female) were subjected to a sham operation (SHAM group) or ovariectomy. OVX rats were treated with DIO (DIO group) or vehicle (OVX group) for 12 weeks. Following treatment, the serum estradiol, bone turnover biomarker levels, and the microarchitecture of tibias were assayed. Based on miRNA microarray and qRT-PCR analyses, differentially expressed (DE) circulating miRNAs were identified between the OVX and SHAM groups (comparison A) and between the DIO and OVX groups (comparison B). Furthermore, putative target genes of shared DE miRNAs with opposite expression trends in the two comparisons were predicted by ingenuity pathway analysis (IPA). Finally, the expression levels of the putative target genes in serum and tibia were validated by qRT-PCR. The micro-CT results demonstrated that DIO had a substantial anti-osteopenic effect on the tibias of OVX rats. In total, we found 5 DE circulating miRNAs (four upregulated and one downregulated) in comparison A and 21 DE circulating miRNAs (15 upregulated and 6 downregulated) in comparison B. However, only one DE circulating miRNA (rno-miR-20a-5p) had opposite expression trends between the two comparisons. Including rno-miR-20a-5p, 7 of the 10 selected DE circulating miRNAs between the two comparisons passed qRT-PCR validation. Specifically, based on qRT-PCR validation, DIO upregulated the expression of rno-miR-20a-5p and downregulated that of three target genes (Tnf, Creb1, and Tgfbr2) of the “osteoclast differentiation” pathway in the tibias of OVX rats. Our results suggested that DIO could change the circulating miRNA profile of OVX rats and inhibited the downregulation of miR-20a-5p in serum and tibia. DIO might exert an anti-osteoclastogenic effect on OVX rats by upregulating the expression of miR-20a-5p in circulation and bone tissue. Frontiers Media S.A. 2020-03-06 /pmc/articles/PMC7069125/ /pubmed/32210807 http://dx.doi.org/10.3389/fphar.2020.00207 Text en Copyright © 2020 Zhang, Xiang, Wang, Jiang, Cheng, Xiao, Ju and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Zhiguo
Xiang, Lihua
Wang, Yuhan
Jiang, Yanhua
Cheng, Yin
Xiao, Gary Guishan
Ju, Dahong
Chen, Yanjing
Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title_full Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title_fullStr Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title_full_unstemmed Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title_short Effect of Diosgenin on the Circulating MicroRNA Profile of Ovariectomized Rats
title_sort effect of diosgenin on the circulating microrna profile of ovariectomized rats
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069125/
https://www.ncbi.nlm.nih.gov/pubmed/32210807
http://dx.doi.org/10.3389/fphar.2020.00207
work_keys_str_mv AT zhangzhiguo effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT xianglihua effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT wangyuhan effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT jiangyanhua effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT chengyin effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT xiaogaryguishan effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT judahong effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats
AT chenyanjing effectofdiosgeninonthecirculatingmicrornaprofileofovariectomizedrats