A multiplex guide RNA expression system and its efficacy for plant genome engineering

BACKGROUND: The Streptococcus pyogenes CRISPR system is composed of a Cas9 endonuclease (SpCas9) and a single-stranded guide RNA (gRNA) harboring a target-specific sequence. Theoretically, SpCas9 proteins could cleave as many targeted loci as gRNAs bind in a genome. RESULTS: We introduce a PCR-free...

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Detalles Bibliográficos
Autores principales: Oh, Youngbin, Lee, Bora, Kim, Hyeonjin, Kim, Sang-Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069183/
https://www.ncbi.nlm.nih.gov/pubmed/32190101
http://dx.doi.org/10.1186/s13007-020-00580-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069183/
https://www.ncbi.nlm.nih.gov/pubmed/32190101
http://dx.doi.org/10.1186/s13007-020-00580-x

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