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A multiplex guide RNA expression system and its efficacy for plant genome engineering
BACKGROUND: The Streptococcus pyogenes CRISPR system is composed of a Cas9 endonuclease (SpCas9) and a single-stranded guide RNA (gRNA) harboring a target-specific sequence. Theoretically, SpCas9 proteins could cleave as many targeted loci as gRNAs bind in a genome. RESULTS: We introduce a PCR-free...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069183/ https://www.ncbi.nlm.nih.gov/pubmed/32190101 http://dx.doi.org/10.1186/s13007-020-00580-x |