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Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea

AIM: This study aimed to investigate the frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea. BACKGROUND: Antibiotic resistance is widespread among diarrheagenic Escherichia coli (DEC) in developing countries. I...

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Autores principales: Farajzadeh-Sheikh, Ahmad, Savari, Mohammad, Hosseini Nave, Hossein, Ahmadi Ahmadi, Khadijeh, Afzali, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069536/
https://www.ncbi.nlm.nih.gov/pubmed/32190228
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author Farajzadeh-Sheikh, Ahmad
Savari, Mohammad
Hosseini Nave, Hossein
Ahmadi Ahmadi, Khadijeh
Afzali, Maryam
author_facet Farajzadeh-Sheikh, Ahmad
Savari, Mohammad
Hosseini Nave, Hossein
Ahmadi Ahmadi, Khadijeh
Afzali, Maryam
author_sort Farajzadeh-Sheikh, Ahmad
collection PubMed
description AIM: This study aimed to investigate the frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea. BACKGROUND: Antibiotic resistance is widespread among diarrheagenic Escherichia coli (DEC) in developing countries. Information regarding Extended-Spectrum β-Lactamases (ESBLs) in diarrheagenic pathogens should be considered in clinical management when an optimal treatment is required. METHODS: A total of 581 stool samples were collected from patients with diarrhea in Ahvaz, Iran. PCR was used for the presence of the ipaH gene to confirm EIEC strains. The antibiotic resistance pattern of all EIEC isolates was determined by the disk diffusion method. EIEC isolates were screened for class A β-lactamase genes. Genotyping of harboring β-lactamase genes was performed by Multi-Locus VNTR Analysis (MLVA). RESULTS: Among 13 EIEC isolates, 9 isolates (69.2%) were found ESBL positive by double-disk synergy test (DDST) and PCR. Furthermore, bla(CTX-M-15) and bla(CTX-M-1) genes were detected in 77.8% (n=7) and 44.5% (n=4) of the bla(CTX-M-1) group. On the other hand, the bla(TEM-1) gene was detected in 66.6% (n=6). None of the isolates had bla(SHV-1), bla(KPC), or bla(GES) genes. Six MLVA genotypes were identified. CONCLUSION: The current study revealed that the presence of ESBLs genes mediates the resistance of EIEC isolates to the majority of antibiotics in this region. The presence of ESBLs genes in different MLVA types showed that one specific clone was not responsible for spreading the EIEC isolates.
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spelling pubmed-70695362020-03-18 Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea Farajzadeh-Sheikh, Ahmad Savari, Mohammad Hosseini Nave, Hossein Ahmadi Ahmadi, Khadijeh Afzali, Maryam Gastroenterol Hepatol Bed Bench Original Article AIM: This study aimed to investigate the frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea. BACKGROUND: Antibiotic resistance is widespread among diarrheagenic Escherichia coli (DEC) in developing countries. Information regarding Extended-Spectrum β-Lactamases (ESBLs) in diarrheagenic pathogens should be considered in clinical management when an optimal treatment is required. METHODS: A total of 581 stool samples were collected from patients with diarrhea in Ahvaz, Iran. PCR was used for the presence of the ipaH gene to confirm EIEC strains. The antibiotic resistance pattern of all EIEC isolates was determined by the disk diffusion method. EIEC isolates were screened for class A β-lactamase genes. Genotyping of harboring β-lactamase genes was performed by Multi-Locus VNTR Analysis (MLVA). RESULTS: Among 13 EIEC isolates, 9 isolates (69.2%) were found ESBL positive by double-disk synergy test (DDST) and PCR. Furthermore, bla(CTX-M-15) and bla(CTX-M-1) genes were detected in 77.8% (n=7) and 44.5% (n=4) of the bla(CTX-M-1) group. On the other hand, the bla(TEM-1) gene was detected in 66.6% (n=6). None of the isolates had bla(SHV-1), bla(KPC), or bla(GES) genes. Six MLVA genotypes were identified. CONCLUSION: The current study revealed that the presence of ESBLs genes mediates the resistance of EIEC isolates to the majority of antibiotics in this region. The presence of ESBLs genes in different MLVA types showed that one specific clone was not responsible for spreading the EIEC isolates. Shaheed Beheshti University of Medical Sciences 2020 /pmc/articles/PMC7069536/ /pubmed/32190228 Text en ©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Farajzadeh-Sheikh, Ahmad
Savari, Mohammad
Hosseini Nave, Hossein
Ahmadi Ahmadi, Khadijeh
Afzali, Maryam
Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title_full Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title_fullStr Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title_full_unstemmed Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title_short Frequency and molecular epidemiology of class A ESBLs producing Enteroinvasive Escherichia coli (EIEC) isolates among patients with diarrhea
title_sort frequency and molecular epidemiology of class a esbls producing enteroinvasive escherichia coli (eiec) isolates among patients with diarrhea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069536/
https://www.ncbi.nlm.nih.gov/pubmed/32190228
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