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The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells

Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue,...

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Autores principales: Jain, Varsha, Murphy-Baum, Benjamin L, deRosenroll, Geoff, Sethuramanujam, Santhosh, Delsey, Mike, Delaney, Kerry R, Awatramani, Gautam Bhagwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069718/
https://www.ncbi.nlm.nih.gov/pubmed/32096758
http://dx.doi.org/10.7554/eLife.52949
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author Jain, Varsha
Murphy-Baum, Benjamin L
deRosenroll, Geoff
Sethuramanujam, Santhosh
Delsey, Mike
Delaney, Kerry R
Awatramani, Gautam Bhagwan
author_facet Jain, Varsha
Murphy-Baum, Benjamin L
deRosenroll, Geoff
Sethuramanujam, Santhosh
Delsey, Mike
Delaney, Kerry R
Awatramani, Gautam Bhagwan
author_sort Jain, Varsha
collection PubMed
description Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue, we took advantage of the retinal direction-selective ganglion cell circuit, where directionally tuned inhibition is known to shape non-directional excitatory signals. We combined two-photon calcium imaging with genetic, pharmacological, and single-cell ablation methods to examine the extent to which inhibition ‘vetoes’ excitation at the level of individual dendrites of direction-selective ganglion cells. We demonstrate that inhibition shapes direction selectivity independently within small dendritic segments (<10µm) with remarkable accuracy. The data suggest that the parallel processing schemes proposed for direction encoding could be more fine-grained than previously envisioned.
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spelling pubmed-70697182020-03-18 The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells Jain, Varsha Murphy-Baum, Benjamin L deRosenroll, Geoff Sethuramanujam, Santhosh Delsey, Mike Delaney, Kerry R Awatramani, Gautam Bhagwan eLife Neuroscience Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue, we took advantage of the retinal direction-selective ganglion cell circuit, where directionally tuned inhibition is known to shape non-directional excitatory signals. We combined two-photon calcium imaging with genetic, pharmacological, and single-cell ablation methods to examine the extent to which inhibition ‘vetoes’ excitation at the level of individual dendrites of direction-selective ganglion cells. We demonstrate that inhibition shapes direction selectivity independently within small dendritic segments (<10µm) with remarkable accuracy. The data suggest that the parallel processing schemes proposed for direction encoding could be more fine-grained than previously envisioned. eLife Sciences Publications, Ltd 2020-02-25 /pmc/articles/PMC7069718/ /pubmed/32096758 http://dx.doi.org/10.7554/eLife.52949 Text en © 2020, Jain et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Jain, Varsha
Murphy-Baum, Benjamin L
deRosenroll, Geoff
Sethuramanujam, Santhosh
Delsey, Mike
Delaney, Kerry R
Awatramani, Gautam Bhagwan
The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title_full The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title_fullStr The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title_full_unstemmed The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title_short The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
title_sort functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069718/
https://www.ncbi.nlm.nih.gov/pubmed/32096758
http://dx.doi.org/10.7554/eLife.52949
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