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The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells
Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069718/ https://www.ncbi.nlm.nih.gov/pubmed/32096758 http://dx.doi.org/10.7554/eLife.52949 |
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author | Jain, Varsha Murphy-Baum, Benjamin L deRosenroll, Geoff Sethuramanujam, Santhosh Delsey, Mike Delaney, Kerry R Awatramani, Gautam Bhagwan |
author_facet | Jain, Varsha Murphy-Baum, Benjamin L deRosenroll, Geoff Sethuramanujam, Santhosh Delsey, Mike Delaney, Kerry R Awatramani, Gautam Bhagwan |
author_sort | Jain, Varsha |
collection | PubMed |
description | Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue, we took advantage of the retinal direction-selective ganglion cell circuit, where directionally tuned inhibition is known to shape non-directional excitatory signals. We combined two-photon calcium imaging with genetic, pharmacological, and single-cell ablation methods to examine the extent to which inhibition ‘vetoes’ excitation at the level of individual dendrites of direction-selective ganglion cells. We demonstrate that inhibition shapes direction selectivity independently within small dendritic segments (<10µm) with remarkable accuracy. The data suggest that the parallel processing schemes proposed for direction encoding could be more fine-grained than previously envisioned. |
format | Online Article Text |
id | pubmed-7069718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70697182020-03-18 The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells Jain, Varsha Murphy-Baum, Benjamin L deRosenroll, Geoff Sethuramanujam, Santhosh Delsey, Mike Delaney, Kerry R Awatramani, Gautam Bhagwan eLife Neuroscience Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue, we took advantage of the retinal direction-selective ganglion cell circuit, where directionally tuned inhibition is known to shape non-directional excitatory signals. We combined two-photon calcium imaging with genetic, pharmacological, and single-cell ablation methods to examine the extent to which inhibition ‘vetoes’ excitation at the level of individual dendrites of direction-selective ganglion cells. We demonstrate that inhibition shapes direction selectivity independently within small dendritic segments (<10µm) with remarkable accuracy. The data suggest that the parallel processing schemes proposed for direction encoding could be more fine-grained than previously envisioned. eLife Sciences Publications, Ltd 2020-02-25 /pmc/articles/PMC7069718/ /pubmed/32096758 http://dx.doi.org/10.7554/eLife.52949 Text en © 2020, Jain et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Jain, Varsha Murphy-Baum, Benjamin L deRosenroll, Geoff Sethuramanujam, Santhosh Delsey, Mike Delaney, Kerry R Awatramani, Gautam Bhagwan The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title | The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title_full | The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title_fullStr | The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title_full_unstemmed | The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title_short | The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
title_sort | functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069718/ https://www.ncbi.nlm.nih.gov/pubmed/32096758 http://dx.doi.org/10.7554/eLife.52949 |
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